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Thermo Fisher Scientific Introduces New Capabilities for Comprehensive Qualitative and Quantitative Proteomics at ASMS 2011

5 Jun 2011
bridget bridget
Laboratory Director

Product news

Thermo Fisher Scientific Inc. today introduced new capabilities for laboratories performing a wide range of qualitative and quantitative proteomics workflows. These capabilities integrate seamlessly with the full portfolio of industry leading solutions offered by Thermo Fisher Scientific, providing laboratories with end-to-end workflows that make a deeper and broader understanding of proteomes and biological pathways possible. The new products can be seen in the Thermo Scientific hospitality suite at the Hyatt Regency in Centennial Ballroom D, during the 59th Annual ASMS Conference on Mass Spectrometry and Allied Topics, from June 5–9 in Denver.

“True realization of comprehensive proteomics is an enormous challenge.” said Andreas Huhmer, proteomics marketing director for life sciences mass spectrometry at Thermo Fisher Scientific. “Overcoming this challenge requires innovative tools that make proteomics analyses more comprehensive in every way. New capabilities, such as our exclusive sample preparation chemistry that targets all kinases in a proteome, will significantly increase the number of proteins scientists can find. Others such as SimGlycan software, offer much more precise characterization of post translational modifications."

Highlighting its commitment to overcoming challenges in proteomics, the company is showcasing:

• Exclusive Thermo Scientific Pierce Kinase Enrichment Kitwith ActivX® ATP or ADP Probes, Thermo Scientific Pierce GTPase Enrichment Kit and Thermo Scientific ActivX Serine Hydrolase Probes that enable the capture and identification of more protein and peptide classes, including kinases which are important to therapeutic drug development research.
• Thermo Scientific Pierce Peptide Calibration Mixture and Thermo Scientific Pierce PEPotec SRM peptide library, for selected reaction monitoring (SRM) assays, to simplify and speed quantitative assays.
• Thermo Scientific EASY-nLC 1000 split-free nano-flow system that significantly increases chromatographic resolution, and thus protein and peptide identifications, with effortless ultra-high pressure operation.
• Innovative mass spectrometers - Thermo Scientific Velos Pro linear ion trap, Orbitrap Velos Pro hybrid FTMS, Q Exactive high performance benchtop quadrupole-Orbitrap LC-MS/MS, and Orbitrap Elite flagship hybrid mass spectrometer – that provide new levels of resolving power, speed, sensitivity and versatility for all proteomics workflows.
• New versions of Thermo Scientific Proteome Discoverer and SIEVE software, as well SimGlycan software from PREMIER Biosoft that enable identification and characterization of more proteins, peptides and post translational modifications (PTMs).
• New version of Thermo Scientific Pinpoint software to enhance the speed and overall confidence in targeted quantitation assays for all experimental approaches including high resolution accurate mass (HRAM) approaches.

More comprehensive protein and peptide characterization for more biological insight

Thermo Scientific is proud to debut products that facilitate every step of qualitative proteomics research from sample preparation and LC-MS analysis, to data processing and integration of information into biological insight. For example, with the company’s exclusive sample preparation chemistries, scientists can capture more protein and peptide classes for subsequent analysis by LC-MS than ever before. These novel chemistries target active serine hydrolases, GTPases, large G-protein subunits and kinases – an important class of pharmaceutical-industry drug targets with promise in cancer treatment.

Updated versions of Proteome Discoverer™, SIEVE™ and SimGlycan® software, leverage the rich, high-quality data produced by Thermo Scientific LC and MS systems and offer new capabilities to enable scientists to explore complex proteomes in much greater depth and breadth, and with greater confidence and speed.

The latest version of Proteome Discoverer software, a comprehensive mass informatics platform, includes features that can be used to determine the location of PTMs, and to mine and integrate biological information, including biological context, from multiple sources. SIEVE software now further speeds interpretation of differentially-expressed proteins and peptides through complete integration with Thermo Scientific ProteinCenter. The software is also expanded to increase the information obtained from metabolomic and lipidomic workflows. SimGlycan software dramatically speeds characterization of complex glycan structures through automated interpretation of MSn data.

Faster, higher-confidence quantitative proteomics

Pinpoint™ software allows scientists to leverage previously acquired discovery data from mass spectrometers, such as the powerful new Q ExactiveTM, to speed and simplify development of robust quantitative assays for all experimental approaches: HRAM, SRM, higher-energy collision induced dissociation (HCD), multiplexed selected ion monitoring (SIM) and multiplexed HCD. The new version includes retention time prediction tools that reduce the number of sample injections needed to ensure high-confidence quantitation results, thereby maximizing throughput. Complementing Pinpoint software, the new Pierce® Peptide Retention Time Calibration Mixture and Pierce® PEPotec SRM custom peptide library speed quantification assays by reducing setup time.

For more information about the new proteomics capabilities, as well as other new Thermo Scientific products, please visit the Thermo Scientific hospitality suite at ASMS 2011. Alternatively, please call visit the company article page.

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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Assay AssemblyAssay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MALDI-TOF MSMALDI-TOF mass spectrometers (matrix-assisted laser desorption/ionization time-of-flight) are used for the mass analysis of large molecules, including proteins, DNA, polymers and other macromolecules due to its relatively soft ionization, reducing fragmentation whilst allowing fast data acquisition. Tandem mass analyzers such as TOF/TOF systems can be used to increase resolution and sensitivity of your analysis. Find the best MALDI-TOF products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :

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Thermo Fisher Scientific Introduces New Capabilities for Comprehensive Qualitative and Quantitative Proteomics at ASMS 2011