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Thermo Fisher Scientific Introduces Kit for Subcellular Protein Fractionation

12 Apr 2009
Veronica Austin
Marketing / Sales

Product news

Thermo Fisher Scientific, the world leader in serving science, introduces a kit for easy and rapid subcellular protein fractionation. The Thermo Scientific Subcellular Protein Fractionation Kit enables the stepwise separation and preparation of cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein extracts from mammalian cultured cells or tissue for protein localization studies or enrichment of specific cellular compartments.

The simple protocol is completed in less than three hours and requires no ultracentrifugation over gradients during any of the steps. The subcellular extracts obtained are compatible with a variety of downstream applications including protein assays, Western blotting, electrophoretic mobility assays and enzyme activity assays.

The Subcellular Protein Fractionation Kit includes four extraction buffers, a stabilized nuclease and the popular Thermo Scientific Halt Protease Inhibitor Cocktail. Each kit has sufficient reagents to fractionate 50 cell pellets of 20 µl each, equivalent to approximately 2 grams of cell paste or tissue. The kit can be stored at -20°C for up to one year. For more information on the Subcellular Protein Fractionation Kit, visit www.thermo.com/pierce or call +1-815-968-0747.

Thermo Scientific is part of Thermo Fisher Scientific, the world leader in serving science.

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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Thermo Fisher Scientific Introduces Kit for Subcellular Protein Fractionation