Thermo Fisher Scientific Introduces Kit for Chromogenic Measurement of Endotoxins in Protein Samples

14 Feb 2012

Product news

Thermo Fisher Scientific, today announced the availability of the Thermo Scientific Pierce LAL Chromogenic Endotoxin Quantitation Kit for the economical and sensitive measurement of endotoxins in protein, peptide or antibody samples. The presence of endotoxins in protein samples can impair downstream analyses and uses.

The Pierce® LAL Chromogenic Endotoxin Quantitation Kit supplies the reagents necessary to generate standard curves and perform endotoxin assays on samples. Reactions are complete in 30 minutes and require only a small volume of diluted protein sample. Absorbance measurements are read at 405 to 410 nanometers using a standard 96-well plate reader. The Pierce LAL Chromogenic Endotoxin Quantitation Kit detects between 0.1 to 1 endotoxin unit per milliliter. The Pierce LAL Chromogenic Endotoxin Quantitation Kit can be used in conjunction with the Thermo Scientific Detoxi-Gel Endotoxin Removal Gel and Pierce High Capacity Endotoxin Removal Resin.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Endotoxins
Thermo Fisher Scientific Introduces Kit for Chromogenic Measurement of Endotoxins in Protein Samples