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Thermo Fisher Scientific Introduces a More Effective Alternative to C18 Resins for Phosphopeptide Clean-up

27 Apr 2010
Sarah Sarah
Marketing / Sales

Product news

Thermo Fisher Scientific introduces the Thermo Scientific Pierce Graphite Spin Columns for the purification of phosphopeptides. The Pierce® Graphite Spin Columns more efficiently bind hydrophilic peptides, such as phosphopeptides, over C18 resins, allowing for the removal of urea, salts and other contaminants.

The columns are ideal for improving mass spectrometric (MS) analyses of samples from protein digests, strong-cation exchange fractions and enriched phosphopeptides eluted from TiO2 and immobilized metal affinity chromatography (IMAC) columns and tips.

With the Pierce Graphite Spin Columns, phosphopeptides can be desalted, concentrated and eluted in 10 minutes, with recoveries of up to 100 micorgrams of hydrophilic peptides per column. The ready-to-use spin column format allows for the simultaneous processing of multiple samples.

The Pierce Graphite Spin Columns are supplied in packages of 30 columns. The columns are easily stored at room temperature and have a shelf life of one year.

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Thermo Fisher Scientific Introduces a More Effective Alternative to C18 Resins for Phosphopeptide Clean-up