Thermo Fisher Scientific Hot Start PCR Reagents Now Available with ReddyMix

Thermo Fisher Scientific Inc., the world leader in serving science, today announced that the Thermo Scientific Thermo-Start® range of hot start PCR reagents is now available with ReddyMix™ buffer for easier, error-free set-up and improved reproducibility of reactions.

The ReddyMix buffer contains an inert red dye, which enhances the contrast between the reagents and the plastic vessel, so that accurate pipetting can be verified easily at a glance. This provides reassurance that each reaction contains the correct volume and enhances the reproducibility of experiments. The red dye and precipitant contained within the ReddyMix buffer also allow the final PCR product to be loaded directly onto agarose gel, reducing post-PCR handling steps and saving valuable time.

Thermo Scientific Thermo-Start Taq DNA Polymerase, Thermo-Start PCR Kits and Thermo-Start Master Mixes, now available with or without ReddyMix, have been developed specifically for hot start PCR applications. The covalently modified enzyme, unlike antibody-mediated enzymes, remains completely inactive until it has been heated to 95ºC for a full 15 minutes, preventing non-specific amplification during reaction set-up and while the PCR reaction heats up.

In addition to general PCR, Thermo-Start reagents are ideal for the amplification of complex genomic or cDNA templates and for low copy-number targets, such as single cell PCR. The reagents are also available with a high-performance buffer for sensitive and specific amplification of challenging targets, such as highly complex and GC-rich templates.

Ben Jackson, product manager, comments, “We aim to provide products that offer unparalleled performance, in formats that are faster and easier to use. The Thermo-Start reagents with Reddymix are important additions to the Thermo Scientific range, offering customers flexibility of choice, greater convenience and enhanced reproducibility of reactions.”

For further information about the wide range of Thermo Scientific products available for PCR, RT-PCR, QPCR and RT-QPCR applications, visit the company article page.