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Thermo Fisher Scientific Expands Popular Line of Thermo Scientific DyLight Fluorescent Dyes and Kits

13 Aug 2009
Veronica Austin
Marketing / Sales

Product news

Thermo Fisher Scientific today introduced the Thermo Scientific DyLight 350 Fluorescent Dye for the direct labeling proteins and other biomolecules. The new dye, a derivative of aminomethylcoumarin acetate (AMCA), fluoresces blue, with excitation and emission wavelengths of 353 and 432 nm, respectively.

The UV-excitable DyLight® 350 Dye is better for multiplexing experiments with green fluorophores over AMCA. It is also more soluble, allowing for higher incorporation of the dye into biomolecules. Its exceptional resistance to photobleaching enables data collection under the most demanding conditions. The DyLight 350 Dye can be measured with common fluorescence instrumentation.

The DyLight 350 Dye is available as an amine-reactive ester or a sulfhydryl-reactive maleimide. Optimized DyLight 350 Antibody Labeling Kits are also available. The standard kit contains all necessary components to perform three individual labeling reactions using 1 mg of IgG or similar protein quantities. A microscale kit contains reagents to perform five separate labeling reactions using 100 micrograms of IgG.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Thermo Fisher Scientific Expands Popular Line of Thermo Scientific DyLight Fluorescent Dyes and Kits