Thermo Fisher Scientific Expands Its Popular Line of Detergent Removal Products

26 May 2011
bridget bridget
Laboratory Director

Product news

Thermo Fisher Scientific, the world leader in serving science, today announced the availability of the Thermo Scientific Pierce Detergent Removal 96-well Spin Plates. The presence of detergents in protein and peptide samples interferes with many downstream analyses such as mass spectrometry (MS). The new spin plates contain the popular Thermo Scientific Pierce Detergent Removal Resin and offer a high-throughput option for efficiently eliminating high concentrations of detergents from protein and peptide samples.

Each well in the Pierce® Detergent Removal 96-well Plate accommodates sample volumes ranging from 25 to 100 microliters. Samples are rapidly processed in 15 minutes with greater than 90 percent protein recovery. The Pierce Detergent Removal Resin has been tested with numerous detergents including Tween®-20, sodium deoxycholate and Triton®X-100.

For more information on the Pierce Detergent Removal 96-well Spin Plates, please visit the company article page.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. 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Thermo Fisher Scientific Expands Its Popular Line of Detergent Removal Products