Thermo Fisher Scientific Adds New Protein Ladders for Accurate Molecular Weight Determination after Gel Electrophoresis

17 Jan 2012

Product news

Thermo Fisher Scientific, today announced the addition of two new protein molecular weight markers for determining the size of proteins in gel electrophoresis and Western blotting applications. The ready-to-use Thermo Scientific PageRuler NIR Protein Ladder contains a mixture of 10 proteins ranging from 11K to 250K. After SDS protein gel electrophoresis or Western blot transfer to a membrane, the protein bands of the marker can be easily visualized using a fluorescent detection instrument, eliminating the need to overlay pre-stained markers. For easy reference, the 55K protein band has greater intensity than the other ladder bands.

The Thermo Scientific PageRuler Unstained High Range Protein Ladder is a mix of eight purified recombinant proteins ranging from 60K to 250K. The ladder is ready to use without heating and diluting. It is easily detected after denaturing protein gel electrophoresis with either coomassie or silver stains, and it can be detected in Western blots with protein stains. The 150K band of the ladder has greater intensity for easy orientation.

For more information about the new PageRuler® NIR Protein Ladder and the PageRuler Unstained High Range Protein Ladder products, please view the company website.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein LadderWeight Marker
Thermo Fisher Scientific Adds New Protein Ladders for Accurate Molecular Weight Determination after Gel Electrophoresis