The VG-FAST96 - a 96-well solution for high throughput screening of PCR products from Scie-Plas

29 Aug 2007

Product news

The VG-FAST96 Vari-gel MAXI system is a fast high throughput solution for molecular genetic studies, allowing up to 96 single-strand polymorphisms (SSPs), 50 to 1000 base pairs in size, to be separated and screened within 30 minutes.

Benefits include: -

  • Six 17-sample combs - enable SSPs to be loaded onto the gel quickly, 8 at a time by multi-channel pipette, while a central marker lane allows both sets of 8 SSPs to be easily distinguished within each row
  • Compact gel tank - minimises the buffer volume required to cover the gel, maximising the voltage gradient and separation speed
  • Robust acrylic gel tray - fits conveniently on standard transilluminators for easy visualisation, while fluorescent numbering aids SNP recognition and traceability
  • Ideal for use - with 96-well microtitre plates or 96-well blocks of the TC-412 and TC-512 Techne® thermal cyclers, also available from Scie-Plas

Specification

  • Gel Size (W x L): 16 x 17.5cm
  • Buffer Volume: 950ml
  • Maximum Sample Capacity: 96 + 8
  • Comb Throughput: 17 samples; 2 x 8 loaded by multi-channel pipette; 1 x marker

VG-FAST96 High Throughput Vari-gel MAXI System

Scie-Plas Ltd

Fast, high throughput microplate compatible electrophoresis The VG-FAST96 high throughput Vari-gel MAXI system is the latest addition to the Scie-Plas Vari-gel Range, providing a swift and simple solution for the screening and analysis of up to 96 single-strand polymorphisms (SSPs) following PCR amplification in 96-well microplates. A single gel tray with UV-fluorescent numbering allows up to 96 SSPs to loaded directly onto the tray, 8 at a time by multi-channel pipette, so that each SSP is automatically numbered for gel documentation, simplifying post-electrophoretic analysis.This unit is ideal for use with 96-well microtitre plates or or the 96-well blocks of the Techne TC412 and TC512 thermal cyclers which are also available through Scie-Plas.VG-FAST96 High Throughput Vari-gel MAXI System Features: Single gel tray with fluorescent numbering - allows up to 96 SSPs to be loaded directly onto the tray, so that each SSP is automatically numbered for gel photography, simplifying postelectrophoretic analysis 6 comb slots - positioned at 25mm intervals along the gel tray allow SSPs to be separated in less than 30 minutes using the appropriate running conditions (see Technical Specification) Six 17-sample combs - with each marker lane positioned centrally - enables SSPs to be loaded onto the gel quickly, 8 at a time by multi-channel pipette Compact tank - reduces the buffer volume required to cover the gel, providing greater control over the voltage gradient and run-time Robust acrylic casting tray – Uvtransparent for visualisation with fluorescent numbering for SNP recognition and traceability Ideal for use - either with 96-well microtitre plates or 96-well blocks Buffer recirculation ports - may be connected to a peristaltic pump for buffer recirculation during electrophoresis

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.SNPs TechnologySingle nucleotide polymorphisms (SNPs) are individual base variations in a DNA sequence. SNPs are used in research to study predispositions to disease and drug discovery. Products for studying single nucleotide polymorphisms include SNP arrays and detection systems to detect polymorphisms, SNP typing systems for genotyping DNA, and PCR to amplify specific SNPs.