The Olympus cellSens Dimension Count & Measure Solution Module for Automated Image Analysis

Olympus has announced the release of its Count & Measure Solution module for the cellSens Dimension software suite. The new module makes it simple to design and automate image analysis protocols by providing advanced object detection and structure differentiation. This maximises accuracy and reproducibility while minimising the time users must devote to manual image processing.

The system can be easily configured to recognise objects and structures based on user specifications by employing a range of threshold-based methods, thereby defining rules with which to process a batch of images. The system is also perfectly suited to performing complex analyses such as those requiring multiple measurements per image.

The new cellSens Count & Measure Solution module integrates seamlessly with the cellSens Dimension platform to supply flexible, automated image analysis. The system provides advanced thresholding and spectral unmixing capabilities to facilitate the easy definition of objects of interest, including automatic, manual, manual Hue Saturation Value (HSV) and adaptive methods. This standardises measurement parameters thereby reducing error and variability, while simplifying and speeding up data capture.

The Count & Measure Solution module makes it easy to create measurement protocols with just a few clicks of the mouse, opening up a range of automated analysis options to users at all levels of expertise. Measurement parameters available include area, aspect ratio, bisector, bounding box, gravity centre, ID, mass centre, intensity values, convexity, diameter, elongation, ferret, extent, next neighbour distance, orientation, perimeter, radius, shape and sphericity. Each of these can be combined and used in conjunction with automatic object detection to perform complex data analysis. This makes it possible to generate comprehensive data sets with minimal user input, all within the same processing run.

cellSens Dimension

The cellSens Dimension software package provides a broad range of advanced features designed to offer maximal control and processing for microscope-based experimental systems. The addition of optional Solution modules, including cellSens Database, Netcam, Count & Measure, Multiposition, Deconvolution and Multichannel 5D, further extends the flexibility and customisability of the platform. Users can conduct a range of complex and highly sophisticated experiments, from extended focal imaging to multiple image alignment and multi-position imaging.

With the ability to control a wide range of Olympus and non-Olympus hardware, advanced and precise time-lapse experiments can be conducted. The resulting time stacks can then be put through kinetic and threshold-based object analysis. The data produced is easy to export and analyse, while the unique report composer uses Microsoft Word templates to generate user-defined reports based on information retrieved directly from the cellSens database. This facilitates interpretation and makes it easy to share and discuss data with colleagues.