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The Latest Cutting Edge Cancer Methods Available for Download

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The SelectScience article library contains downloads on the very latest technologies being used in cancer research and diagnostics

10 Feb 2016
Sonia Nicholas
Managing Editor and Clinical Lead

Editorial article

cancer diagnostics

The SelectScience application article library is updated daily with new case studies, white papers, scientific posters and application notes

Scientific advances in the field of cancer diagnostics are rapidly progressing. This article highlights some of the most interesting new articles available to download from the SelectScience library including topics such as next generations sequencing and liquid biopsies.

1. Somatic Variant Detection from Liquid Biopsy Samples using Targeted NGS

This application note describes a robust method for detecting somatic mutations from liquid biopsy samples by combining circulating tumor cell (CTC) enrichment with a high sensitivity targeted next-generation sequencing (NGS) panel. The combined workflow has broad implications for how cancer can be monitored non-invasively using a routine blood draw without the need for tissue biopsies.

2. Fast and Reliable Real Time PCR Quantification of Leukemia Associated Genes

The aim of this study was to automate the quantification of gene expression in leukemia cells focusing on 140 genes that affect telomere length, apoptosis control, NF-kB pathway and protein degradation. The study used samples for patients with acute myelogenous leukemia (AML) and acute lymphoblastic leukemia (ALL) at different stages of progression and treatment.

3. Correlation of Mutations Detected in Liquid and Tissue Biopsies Using the Maxwell® RSC

This application note describes a method for purifying nucleic acids using the Maxwell® RSC instrument, based on novel surface and binding chemistries. Circulating cell-free DNA (ccfDNA) in plasma can be used to detect biomarkers that show great promise for diagnosis and monitoring of cancer, giving rise to the possibility of liquid biopsies that obviate the need for invasive tissue collection.

4. Circulating Cell-Free DNA and Circulating Tumor Cell Detection on the MassARRAY® System

Circulating cell-free DNA (ccfDNA), isolated from the plasma portion of a blood draw, can originate from apoptotic and necrotic tumor cells, whereas circulating tumor cells (CTCs) are living cells that have shed from the primary tumor and circulate in the bloodstream. This application note answers some frequently asked questions regarding ccfDNA and CTCs and demonstrates the application of the MassARRAY® system in their study through the use of case studies.

5. Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System

In this application note, the SNAP i.d.® 2.0 Protein Detection System for multi-slide screening with immunohistochemistry (IHC) to perform screening of an extensive panel of different breast cancer patient samples and other non-breast cancer tissue microarray (TMA) slide samples to determine their relevance to disease.

6. A Solution for The High Content Screening Analysis of Neutrophil Chemotaxis Assays

In this document BellBrook Labs describe studying neutrophil migration using an iuvo Chemotaxis Assay plate with TTP LabTech’s Acumen eX3 high content imager, and inverted Nikon Eclipse TE2000U microscope is also used for image acquisition.

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HematologyIn Haematology / Hematology, complete blood cell counts (or full blood counts) are obtained using automated blood count analyzers to enumerate blood cell types.  Hematology also encompasses haemostasis and coagulation, thrombophilia and hemophilia, plasma viscosity and ESR analysis, hemoglobinopathies, cell morphology and haematinic measurement.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Cellular PathologyCellular Pathology deals with the microscopic analysis of tissue samples and cells. Sample preparation and processing includes fixation, staining, sectioning and slide mounting, using equipment such microtomes and cryostats. In choosing immunohistochemistry and immunocytochemistry kits, consider chromogens, staining method, antibodies, microscopes and imaging.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Point-of-CarePoint-of-Care Testing (POCT) or Near Patient Testing (NPT) products are available for urine, blood and other clinical chemistry analyses. POCT includes: blood glucose testing, blood gas and electrolytes analysis, rapid coagulation testing (PT / INR), rapid cardiac markers diagnostics, drugs of abuse screening, urine strips testing, pregnancy testing, fecal occult blood analysis, food pathogens screening, hemoglobin diagnostics, infectious disease testing and cholesterol screening.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Clinical ChemistryBiochemistry (or clinical chemistry) involves the analysis of bodily fluids using chemical tests. Techniques used include HPLC, chromatography, spectroscopy, mass spectrometry, immunochemical, electrophoresis, turbidometric / spectrophotometric assay, MRI and ISE analysis. Tests are often carried out on plasma or serum but urine (urinalysis) and fecal specimens are also processed.Clinical GeneticsMolecular Genetics covers the analysis of hereditary genetic disease and chromosomal abnormalities. Genetics can be analysed using DNA, RNA, and protein microarrays, PCR, RT PCR and DNA sequencing. Genetic equipment includes genetic workstations, thermal cyclers, cooling blocks and electrophoresis products. Diagnostic kits are used for DNA / RNA extraction and purification.Cancer DiagnosticsThere are a wide variety of diagnostic tests for cancer available, and this range continues to expand as our knowledge of cancer improves. Current diagnostic methods include biopsy, imaging and blood tests for known biomarkers. New methods in research development include liquid biopsies and cancer breathalyzers.Clinical NGSNext Generation Sequencing (NGS) refers to the use of massive parallel sequencing of multiple small fragments of DNA. This high-throughput genomic analysis yields enormous amounts of sequence data, which if appropriately analyzed could have huge potential for clinical laboratories. For this to happen there are technique and bioinformatic hurdles to be overcome.Liquid BiopsiesLiquid biopsies are non-invasive tests used to detect cancer and other diseases by analyzing blood or other bodily fluids. This technique is gaining popularity for early detection, monitoring treatment response, and detecting minimal residual disease. Explore liquid biopsy solutions in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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The Latest Cutting Edge Cancer Methods Available for Download