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The future of CIEF-mass spec techniques for protein characterization

SelectScience

Watch this webinar to find out how directly coupling CIEF with mass spectrometry can benefit biopharmaceutical applications

8 Jun 2020
Laura Sisman
Administrator / Office Personnel

Expert insights

Dr. Carsten Sönksen (top left), Dr. Tiemin Huang (bottom left), and Dr. Christian Neusüß (right)

The CEInfinite enables highly efficient charged-based separation and direct MS coupled analysis using cIEF. It allows for unattended high resolution fractionation of your charge variants for further analysis, such as MS characterization, SPR and Taylor dispersion protein hydrodynamic radius measurement.

In this webinar, Prof. Dr. Christian Neusüß (Aalen University) and Dr. Carsten Sönksen (Novo Nordisk) discuss the results of their work on online iCIEF-MS of intact monoclonal antibody samples and analysis of fractions collected from iCIEF separated protein. This is truly the first easy online direct coupling of iCIEF to MS providing direct identification of each intact mAb charge variant. This method provides unattended high resolution fractionation of such variants for further offline characterizations like peptide mapping.

Register here

  • Prof. Dr. Christian Neusüß (Aalen University) will share insights into the application of iCIEF-MS for protein isoform characterization.
  • Dr. Carsten Sönksen (Novo Nordisk) will present data using prep-iCIEF with subsequent peptide mapping and LC-MS analysis.
  • Dr. Tiemin Huang (President & CEO, Advanced Electrophoresis Solutions) will precede the presentations explaining the experimental setup needed to run such experiments.

You will learn about:

  • Routine protein isoform characterization by MS and peptide mapping, using either direct MS coupling or high resolution fractionation
  • How to make routine unattended CIEF fractionation possible
  • Whole solution charge-based protein separation and characterization using CIEF

Who should attend:

  • Scientists working in assay development or biopharmaceutical QC labs, including CROs and CMOs
  • Anyone who works with CIEF, IEX, Gel-IEF, or anything with iso electrical focusing

Certificate of Attendance

All webinar participants can request a certificate of attendance, and a learning outcomes summary document for continuing education purposes.

Attend the webinar on Tuesday, June 23, at:

  • 16:00 BST
  • 11:00 EDT
  • 08:00 PDT
  • 17:00 CEST

Register for this webinar here>>

This webinar is part of the SelectScience Advances in Mass Spectrometry Webinar Series >>

SelectScience runs 3-4 webinars a month across various scientific topics, discover more of our upcoming webinars>>

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.Peptide MappingAssay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.Webinar

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