The epMotion 5073m NGS Solution for Economical Library Construction

Eppendorf announces solution to constructing next-generation sequencing (NGS) libraries in cases of small sample numbers and limited laboratory space

8 May 2019
Charlie Carter
Life Sciences Editor

Product news

Until now, the automated construction of next generation sequencing libraries has only been economical for preparations of at least 96 samples, as entire plates are processed using multichannel dispensing tools.

Furthermore, large pipetting stations are often costly and take up valuable space in the laboratory. As a result, smaller sample numbers are processed manually using pipettes. This approach requires time and concentration, and it involves a multitude of pipetting steps. Pipetting errors are difficult to avoid entirely, and they are rarely traceable. At the same time, NGS libraries, and the quality of their sequences, rely on the accuracy and reproducibility afforded by automated pipetting stations.

A solution is now available for all researchers who work with small sample numbers, whose laboratory space is limited or who generate NGS libraries manually. Up to 24 samples can be processed simultaneously in a fully automated fashion using the epMotion® 5073m NGS solution. The work station is optimized in such a way that almost no intervention is required and the construction of the library can proceed independently. Smooth operation is guaranteed by ample space for a sufficient number of pipette tips, the three required single and multichannel pipetting tools in the volume range covering 5-300 µL, and a special rack that accommodates all the required solutions.

The renowned precision and accuracy of Eppendorf pipettes also applies to the dispensing tools within the epMotion. The optimized epBlue™ software enables smart use of the single channel and multichannel dispensing tools in order to optimize pipetting steps and tip use even in the case of odd sample numbers. This saves time and money, and it reduces waste. And all of that on a footprint of only 6 letter-sized pieces of paper. Notification emails generated by the system to alert the user about manual steps allow the operator to step away from the instrument and focus their attention on other tasks.

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epMotion® 5070

Eppendorf

  Our smallest member of the epMotion family is the most compact solution for accurate and reproducible automated pipetting. This makes the epMotion 5070 a perfect match for any routine application such as PCR and qPCR set up, serial dilutions, reagent distribution, sample transfer from tubes to plates, and sample normalization.

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epMotion® 5075

Eppendorf

 The epMotion 5075 is the ideal solution for diverse liquid handling demands. It offers the same outstanding accuracy and precision as epMotion 5070 and 5073. The available options make the 5075 an excellent and flexible device for applications such as NGS library preparation, real-time PCR set-up, magnetic bead-based and filter-based purification, cell-based assays or any routine pipetting tasks.

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epBlue ID: Secure Barcode Scanning - Tracking - Documentation

Eppendorf

With epMotion® you are able to automate all routine liquid handling tasks. Together with the epBlue ID software the epMotion can now be employed in application areas that require process documentation. The new module of the epBlue software system allows simple and safe data exchange with your laboratory information management system by using all common barcodes. Barcoded samples, plates and reagents will be scanned manually and their origin identified and the process documented. epBlue ID will create a result file after the automated process and present it to a laboratory database for safe documentation.

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Pipettes and DispensersPipettes and dispensers are essential lab equipment used to dispense measured volumes of liquid accurately. Explore a range of manual and electronic pipettes, including single and multichannel, serological and volumetric pipettes, as well as a range of pipette tips. Bottle-top dispensers are also available for simple and fast solvent dispensing. Find the best pipettes & dispensers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Library GenerationLibrary generation refers to the construction of NGS libraries from RNA and DNA sources.Pipette TipsPipette tips are essential accessories used in laboratory settings for transferring precise volumes of liquids. They come in various sizes and materials to accommodate different types of samples, ensuring accuracy and contamination prevention. Browse our peer-reviewed product directory to find the best pipette tips for your laboratory needs, compare products, check reviews, and get pricing directly from manufacturers.
The epMotion 5073m NGS Solution for Economical Library Construction