ResourceLife Sciences
The complete guide to stain-free Western blotting
29 May 2025Learn how stain-free western blotting will revolutionize your research
This guide will bring you up to speed on the advantages of Stain-Free technology. By reading this guide you will learn:
- The benefits of Stain-Free Technology
- How to save 11 hours with stain-free vs the traditional western blotting protocol
- Why you should always be using Total Protein Normalization
- How to ensure your blots are publishable
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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.