Techne PrimeQ Opens Up New Flexibility for Real-Time PCR

Designed with all the advantages of an open architecture and chemistry format, PrimeQ is the new real-time PCR system from Techne. Supported by Quansoft software, PrimeQ gives users the freedom to choose their preferred chemistry, consumables and data analysis methods.

PrimeQ has been designed with a single white LED light source, a photomultiplier tube (PMT) detector providing accurate raw data for analysis and a flexible scanning mechanism allowing both partial and full plate reads. With five paired excitation and emission filters covering wavelengths 470–710nm and housed in an individual cartridge system, PrimeQ has the capability for multiplex applications.

Quansoft gives users the flexibility to manipulate raw data, select chemistries and choose consumables. This comprehensive analysis package is easy to use and experimental setups can be edited even after the run is complete. Quansoft is free and can be loaded on multiple PCs without paying a license fee.

According to Rob Skehens, Bibby Scientific Marketing Director, ‘Techne aims to enhance the user experience across its entire thermal cycler range. PrimeQ introduces Quansoft, a unique, intuitive software package. Its four user-friendly editor functions and clear color-coded graphics make the process of creating and analyzing real-time PCR experiments much simpler and faster.’

Using intercalating dyes or probes, PrimeQ provides a wide dynamic range and high sensitivity for absolute and relative quantification. Other applications include dissociation analysis for genotyping experiments and product verification, as well as end point analysis for plus/minus scoring and allelic discrimination.