Tecan’s Freedom EVO® Meets the Demands of Automated LC-MS Sample Preparation

30 May 2011
bridget bridget
Laboratory Director

Product news

Tecan, one of the world’s leading players in laboratory automation, will be highlighting its Freedom EVO liquid handling platform and accessories, demonstrating the system’s proven capability to handle high sample throughput and meet the ever increasing demand for rapid sample preparation for LC-MS analysis.

The growing number of samples being analyzed by LC and LC-MS has resulted in a significant increase in the time spent by laboratories on sample preparation. Manual sample preparation is extremely time consuming, and laboratories have increasingly turned to automated sample preparation to meet high throughput demands and quality requirements. Tecan’s flexible Freedom EVO liquid handling platforms provide connectivity to downstream solutions and data can be transferred between the instrument and the laboratory’s LIMS, offering fully automated, high throughput sample preparation for LC-MS analysis.

Visitors to the booth will be able to see the flexible Freedom EVO workstation and learn how it can be configured to meet their particular needs. The system is available in four different worktable capacities, with a range of liquid handling and robotic manipulator arms that enable precise and reliable automation of advanced liquid handling tasks, and can be easily reconfigured to evolve with changing application requirements, providing long-term protection of your laboratory’s investment.

The highest degree of process and data integrity is guaranteed by the Freedom EVO’s safety and detection features, including capacitive- and/or pressure-based detection and monitoring of sample liquid levels during pipetting, as well as a Pressure Monitored Pipetting (PMP™) option to ensure a complete record of any errors. Fully automated solid phase extraction can be performed using the Te­VacS™ module, and complete sample tracking is accomplished via the PosID™ barcode scanner. The platform’s intuitive, easy-to-use Freedom EVOware® software provides total control of even the most complex applications, allowing easy implementation of methods while maximizing system performance.

For more information, visit Tecan at booth #153.

Tecan Freedom EVO® Workstation

Tecan

The Freedom EVO platform offers fully automated liquid handling and robotics for life sciences and diagnostics. This flexible system offers virtually unlimited options, which can be tailored to provide individual solutions.

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PosID™ Positive Identification System

Tecan

The PosID (Positive Identification System) enables barcodes on standard labware such as tubes, microplates, deep-well plates, and gel cards to be decoded prior to and during processing. System components can be used to complement security requirements in any application in Clinical Diagnostics or in Biopharma research. The PosID is mounted at the rear of the worktable on Freedom EVO® 100, 150 or 200 platforms. The module can identify barcodes mounted on labware carriers, identifying in the first instance which labware type is present on the carrier. The unique pull-back mechanism then automatically moves the carrier across the path of the barcode scanner which orientates itself automatically to decode barcodes mounted vertically or horizontally on microplates, tubes, gel cards or reagent troughs. The PosID can indicate if a position is empty, for example a sample tube position that has not been loaded will receive the status “no tube” and will not be processed. A barcode that is obscured or missing will be logged as unidentifiable and not processed. The PosID offers users easy, additional process control with its ability to indicate to the operator, at a glance, which racks have been processed and may be removed by shifting these carriers to a unique position at the front of the worktable. The barcode scanner is capable of decoding a broad range of code types and densities, in fact more than 20 code types normally found in the laboratory. The entire Tecan Positive Identification concept comprises any combination of the following options: PosID – a robotic barcode scanner mounted at the rear of the Freedom EVO with free range to monitor labware carrier positions and to automatically identify the codes of numerous labware types. Barcode Reader – a stand-alone barcode reader scans codes on labware during transfer actions by the Tecan Robotic manipulator. Logistic barcode reader – scans codes automatically on labware at the loading ports of Tecan’s high speed Carousel HS™ and Stacker modules.

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Te-VacS™ vacuum separation module

Tecan

The Te-VacS system is a versatile vacuum separation module for Tecan pipetting platforms. It is designed to fully automate cartridge-based filtration and/or solid phase extraction (SPE) processes. Te-VacS is able to host different types of SPE plates and SPE cartridges from various suppliers. It can be operated singly or in combination with other modules on Tecan pipetting platforms thereby eliminating cost-intensive hands-on time by lab personnel in the life science industry. The Te-VacS module provides two separate vacuum positions connected to a high performance vacuum pump. The module can easily be integrated into Freedom EVO® workstations. No external power supply is necessary. Straightforward operating and control of the device is enabled by Tecan’s Pipetting Software Packages. Te-VacS highlights: Te-VacS can easily be configured to host different types of SPE plates and SPE cartridges from various suppliers. Separate vent lines for each vacuum position prevent aerosol-caused cross-contamination. Automated exchange of tubes or plates on the Te-VacS allows multiple batch processes without user intervention. Process scale-up can be realized by parallel use of multiple Te-VacS systems on one single workstation. Te-VacS technology is compatible with vacuum-based reagent kits from numerous vendors such as Millipore®, Promega®, MACHEREY-NAGEL and others. Te-VacS applications: Extraction of plasmid DNA Purification of PCR products and cycle sequencing products Purification of genomic DNA from blood, body fluids, cells, tissue, micro-organisms, food or plants Isolation of viral nucleic acids from blood plasma or serum Extraction of drugs from blood plasma Filtration and ultra-filtration

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.GC-MS GC-MS (gas chromatography-mass spectrometry) instruments and equipment are used to separate, quantify and identify mixtures of small and volatile compounds, such as polycyclic aromatics, fatty acids and alcohols. Often used in drug detection, forensic investigation and environmental analysis for pesticides and contaminants, GC-MS is a powerful addition to your lab’s analytical capabilities. GC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzers can give greater sensitivity and resolution to your analysis. Find the best GC-MS instruments and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.