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Tecan Offers Next-Generation Sequencing Sample Preparation at the Touch of a Button

Tecan
17 Sept 2014
Sarah Thomas
Associate Editor

Product news

Tecan has launched the Freedom EVO® NGS workstation to simplify next-generation sequencing (NGS) sample preparation and PCR set-up. Offering user-friendly, walkaway automation of library preparation at the touch of a button, it eliminates the need for extensive manual processing.

NGS is the fastest growing application in genomics, yet many sample preparation protocols can be labor-intensive and time-consuming. The Freedom EVO NGS workstation has been designed to offer robust and reliable automation – including library preparation, quantification, qPCR set-up, normalization, pooling and capture – even for inexperienced users. This preconfigured system includes all the modules required for precise set-up of NGS sequencing libraries, reducing hands-on time and increasing productivity for NGS applications.

Developed in collaboration with the High-Throughput Sequencing Facility, University of North Carolina at Chapel Hill, the compact system offers one-touch protocol selection and step-by-step user instructions via Tecan’s intuitive TouchTools™ graphical interface. It is supplied with ready-to-run protocols for popular NGS technologies – including Illumina-qualified* protocols for the TruSeq® Stranded mRNA Library Prep Kit and Nextera® Rapid Capture Exome Kit – helping you to hit the ground running and simplifying day-to-day activities.

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Freedom EVO® NGS Workstation

Tecan

The Freedom EVO NGS workstation combines Tecan’s expertise in laboratory automation with sequencing-verified protocols to offer hassle-free NGS sample prep at the touch of a button. NGS is the fastest growing application in genomics, yet many sample preparation protocols can be labor intensive and time consuming. The Freedom EVO NGS workstation has been developed to offer robust and reliable automation of library preparation, quantification, qPCR set-up, normalization, pooling and capture. Offering user-friendly, walkaway automation at the touch of a button, it eliminates the need for extensive manual processing, helping to simplify day-to-day activities and ensure high quality results. Freedom EVO NGS workstation includes all the modules required for precise set-up of NGS sequencing libraries and, with one-touch method selection and step-by-step user instructions via Tecan’s intuitive TouchTools™ graphical interface, it reduces hands-on time and increases productivity – even for inexperienced users. The system’s key benefits include: Precise pipetting – from 1,000 µl down to 0.5 µl using eight pipetting channels and innovative air displacement pipetting technology Integrated temperature control – to keep reagents cool and provide optimal enzymatic conditions Efficient bead clean-up –  using a 96-position magnetic plate separator (Alpaqua 96S Super Magnet Plate) Walkaway processing – with on-board consumables storage for long unattended runs Straightforward operation – with step-by-step set-up instructions To further streamline processing, Tecan has worked with leading industry partners to supply ready-to-run protocols for a number of popular NGS technologies. Protocols for Illumina’s TruSeq™ Nano DNA, TruSeq Stranded mRNA* and Nextera™ Rapid Capture Exome* chemistries have been developed in collaboration with Illumina and the High-Throughput Sequencing Facility, University of North Carolina at Chapel Hill, with thorough testing and sequencing data analysis to ensure high quality results. In addition, the system is supplied with protocols covering the Ion Torrent™ library preparation workflow for Ion AmpliSeq™ panels, developed and supported by Thermo Fisher Scientific. *Illumina-qualified protocol.

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Tecan Offers Next-Generation Sequencing Sample Preparation at the Touch of a Button