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Tecan launches rapid viral and pathogen characterization RNA-Seq library preparation solution for challenging sample types

Tecan

The kit is ideally suited to NGS library preparation from a low input, patient-derived sample types, such as nasal swabs

4 Dec 2020
Diane Li
Assistant Editor

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Tecan has launched the Revelo RNA-Seq kit, offering end-to-end processing of human samples to enable complete viral RNA-Seq library preparation – including library quantification – in a single day. This kit has been developed specifically to address the pathogen detection and characterization challenges currently faced by laboratories around the world, and is ideally suited to NGS library preparation from low input, patient-derived sample types, such as nasal swabs.

Revelo RNA-Seq offers a user-friendly solution for cost-effective, same-day library preparation by combining an enhanced version of Tecan’s SPIA® (Single Primer Isothermal Amplification) technology with the company’s unique, fluorescence-based NuQuant® library quantification. Optimized to provide high sensitivity for degraded or low copy number viral RNA in human samples, it allows rapid, sensitive identification and characterization of pathogens. This new kit has already been rigorously tested with a range of patient-derived samples containing pathogens, such as SARS-CoV-2 and other respiratory diseases. The automation-friendly kit has also been implemented on Tecan’s DreamPrep™ NGS workstation to offer a complete, automated solution for walkaway processing, helping to increase throughput and efficiency in busy labs.

Klaus Lun, Executive Vice President and Head of the Life Sciences Business Division at Tecan, commented: “The Revelo RNA-Seq kit was developed in response to an urgent need for faster, easier pathogen library preparation from low copy number or degraded sample types, such as nasal swabs. Optimized for enhanced human rRNA depletion, the kit offers ‘SPIA with a boost’ to ensure better sensitivity and reduced sequencing costs.”

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Freedom EVO® NGS Workstation

Tecan

The Freedom EVO NGS workstation combines Tecan’s expertise in laboratory automation with sequencing-verified protocols to offer hassle-free NGS sample prep at the touch of a button. NGS is the fastest growing application in genomics, yet many sample preparation protocols can be labor intensive and time consuming. The Freedom EVO NGS workstation has been developed to offer robust and reliable automation of library preparation, quantification, qPCR set-up, normalization, pooling and capture. Offering user-friendly, walkaway automation at the touch of a button, it eliminates the need for extensive manual processing, helping to simplify day-to-day activities and ensure high quality results. Freedom EVO NGS workstation includes all the modules required for precise set-up of NGS sequencing libraries and, with one-touch method selection and step-by-step user instructions via Tecan’s intuitive TouchTools™ graphical interface, it reduces hands-on time and increases productivity – even for inexperienced users. The system’s key benefits include: Precise pipetting – from 1,000 µl down to 0.5 µl using eight pipetting channels and innovative air displacement pipetting technology Integrated temperature control – to keep reagents cool and provide optimal enzymatic conditions Efficient bead clean-up –  using a 96-position magnetic plate separator (Alpaqua 96S Super Magnet Plate) Walkaway processing – with on-board consumables storage for long unattended runs Straightforward operation – with step-by-step set-up instructions To further streamline processing, Tecan has worked with leading industry partners to supply ready-to-run protocols for a number of popular NGS technologies. Protocols for Illumina’s TruSeq™ Nano DNA, TruSeq Stranded mRNA* and Nextera™ Rapid Capture Exome* chemistries have been developed in collaboration with Illumina and the High-Throughput Sequencing Facility, University of North Carolina at Chapel Hill, with thorough testing and sequencing data analysis to ensure high quality results. In addition, the system is supplied with protocols covering the Ion Torrent™ library preparation workflow for Ion AmpliSeq™ panels, developed and supported by Thermo Fisher Scientific. *Illumina-qualified protocol.

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