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TaqMan<sup>®</sup> MicroRNA Megaplex™ Products

Thermo Fisher Scientific
23 Jun 2008

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The TaqMan® MicroRNA MegaPlex products consist of three new molecular tools designed to streamline the workflow for microRNA (miRNA) analysis, address the needs of researchers working with minute amounts of RNA, and provide broad, up-to-date coverage of known miRNAs expressed in biological samples from humans, mice, and rats.

The Megaplex™ RT Primers consist of high complexity pools of novel stem-looped reverse transcriptase (RT) primers that reduce the number of RT reactions needed to profile miRNA expression. The optional Megaplex™ PreAmp Primers enable pre-amplification of starting RNA material when sensitivity is of the utmost importance. Incorporation of pre-amplification reduces the total RNA input needed to perform an analysis to as little as one nanogram. The TaqMan® MicroRNA Arrays are microfluidics cards containing TaqMan MicroRNA Assays that enable rapid and reproducible generation of real-time PCR data for hundreds of miRNA targets in parallel.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.

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TaqMan<sup>®</sup> MicroRNA Megaplex™ Products