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Take EMD Millipore’s Research Survival Challenge at Neuroscience 2014

Merck
3 Nov 2014
Sarah Thomas
Associate Editor

Industry news

Visit EMD Millipore at Neuroscience 2014, the premier venue for neuroscientists to debut cutting-edge research on the brain and nervous system. At the meeting, scientists gather to facilitate the integration of research directed at all levels of biological organization and developing improved therapies.

Activities at EMD Millipore’s Booth (#1623) include:
• Issues with your tissues? Check out the NEW SNAP i.d.® 2.0 system for immunohistochemistry
• Sign up to receive your 15-month Neuroscience Calendar
• Receive a copy of EMD Millipore’s popular neuroinflammation pathways wall poster
• Take the Research Survival Challenge — Enter to win a 8.9" HDX tablet*
• Let EMD Millipore’s booth artist draw your caricature — you'll be inspired!

You can also attend EMD Millipore’s research poster presentations, which cover a range of applications and techniques including iPSCs generation, proginator cell culture, and live cell mRNA detection technology.

Tue, Nov 18, 1:00 - 5:00 PM
590.03/A35 - Single transfection of a synthetic polycistronic self-replicative RNA yields human iPSCs that can be efficiently expanded & directed to neural progenitor cells

Tue, Nov 18, 1:00 - 5:00 PM
590.28/A60 - Efficient generation of NKX2.2 and OLIG2 positive progenitor cells from human pluripotent stem cells in defined serum free culture

Tue, Nov 18, 1:00 - 5:00 PM
657.22/UU87 - Identification of astrocytes derived from neural progenitor cells by high-throughput screening using a live cell mRNA detection technology

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SNAP i.d. 2.0 System

Merck

The SNAP i.d. Protein Detection System revolutionizes the quality of your blots every time — in record time! Unlike conventional Western blotting, where diffusion is the primary means of reagent transport, the SNAP i.d. system applies a vacuum to actively drive reagents through the membrane. This novel method allows you to optimize your blotting conditions in record time for maximum results. The SNAP i.d. system minimizes overblocking by using lower concentrations of blocking agents. In addition, more effective wash steps remove unwanted contaminants from the membrane. With the SNAP i.d. system, you’ll achieve incredibly low background, high signal-to-noise ratios, reproducibility from blot to blot, and sensitivities that are the same or better than traditional immunodetection techniques. Compatible with your favorite membrane and detection methods, the SNAP i.d. system produces higher quality blots than traditional western blot methods -- in less than 30 minutes. SNAP i.d. System Features: Dynamic – vacuum actively drives reagents through blotting membrane. Quality – equal or better signal-to-noise ratios than standard western blotting. Fast – reduces immunodetection time from 4 hours to 30 minutes. Simple – incorporates blocking, washing, and antibody incubation steps. Compatible – works with standard gel sizes and protocols. Efficient – optimizes your protocol with a new antibody in 30 minutes.

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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :NeuroscienceNeuroscience research investigates the neurological mechanisms underlying behavior, neurodegenerative diseases, and other brain conditions. Learn about the innovative technologies for bioimaging, electrophysiology, cell culture, chromatography and other techniques used in this field.ImmunohistochemistryImmunohistochemistry (IHC) is a technique used to detect specific proteins in tissue samples by using antibodies that bind to target antigens. IHC is widely applied in pathology, immunology, hematology and cancer diagnostics. Explore IHC tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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Take EMD Millipore’s Research Survival Challenge at Neuroscience 2014