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Standardisation of Direct Detection of DNA Damage In Cells…

AMSBIO
27 Sept 2009
Sarah Sarah
Marketing / Sales

Product news

Amsbio has announced a novel complete assay system for direct detection of DNA damage in cells which includes CometSlides™, reagents, control cells and an electrophoresis unit. The comet assay is the only direct method for the detection of DNA damage in cells.

It is used in cancer research, in genotoxicity studies on environmental mutagens, and for screening compounds for cancer therapeutics. Based on single cell gel electrophoresis in a controlled pH environment, the assay allows the integrity of stained nuclear DNA to be examined and measured.

Until now, a lack of standardisation in electrophoresis equipment, control cells, comet slides and comet reagents created inconsistencies in data generated run-to-run, user-to-user, and lab-to-lab. The new complete assay system from amsbio solves these problems.

The complete assay system includes CometSlides™, reagents, control cells and an electrophoresis unit which retains test cells in a uniquely configured electrophoretic field permitting consistent DNA migration patterns, which are critical for standardisation of the assay. Each lot of the CometAssay™ control cells, reagents and CometSlides™ are tested and qualified for use in the CometAssay™ System.

In combination, the components of the system permit reproducibility and reduce variability between individual users and different laboratories.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Standardisation of Direct Detection of DNA Damage In Cells…