SpectraPlex Fluorescent Western Blot Kit: Get more data from each western blot with fluorescent detection.

SpectraPlex™ provides scientists with the tools needed to perform quantitative, two-color fluorescent western blots. With multicolor detection, it is possible to simultaneously quantify a loading control and a protein of interest, or phosphorylated isoforms of a single protein, greatly increasing the information from as single blot while saving time and reagent costs.

The SpectraPlex™ kit includes fluorescently labeled secondary antibodies, and blocking and washing buffers optimized to work together to provide high sensitivity; as little as 1 pg of protein can be detected.

Researchers currently performing chemiluminescent westerns should find the transition to fluorescence easy, since the SpectraPlex protocol is very similar to those used for chemiluminescent detection.

The excitation and emission spectra of the SpectraPlex secondary antibodies are compatible with common fluorescence imaging systems.

SpectraPlex was developed for the FluorChem® Q, a western blot imaging station for both fluorescent and chemiluminescent detection which comes with imaging and analysis software specifically designed for quantitative analysis of western blots. Together, SpectraPlex and the FluorChem® Q provide a complete platform for fluorescent western blotting.