Spark Holland’s Reliance™ autosampler proves to be a true bio-injector

24 May 2007

Product news

Spark Holland has been operating at the forefront of autosampler design for 25 years. They evolved into a leading OEM autosampler supplier and the leading on-line SPE provider. Spark’s Symbiosis SPE system is used around the world for biological sample processing. An essential part of this system is the Reliance autosampler that is also being used by customers as a stand-alone version for bioanalysis.

Due to many enthusiastic responses about the carry-over performance, Spark will now start promoting the Reliance as a stand-alone autosampler. In many cases customers have decided to run the more challenging compounds on the Reliance so that they do not waste valuable time on reducing autosampler carry-over. Several head to head carry-over comparisons with other autosamplers have indeed shown the Reliance being the top performing autosampler.

A recent comparison was performed by Nicholas Deagon at Charles River Laboratories, Preclinical Services Montreal who concludes: “Our evaluation of the Reliance, both in LC and on-line extraction modes, demonstrated this autosampler to have the potential to surpass the carry-over performance of other popular autosamplers used in the field of quantitative bioanalysis.” Other examples of head to head comparisons show similar results.

With the Reliance autosampler Spark is aiming for customers looking for an autosampler that meets the performance standards required in bioanalysis today. Especially with the increasing detection limits of MS systems, carry-over performance of the autosamper has become even more important. To support the promotion of the Reliance, several instruments will be made available that can be used as demo units for testing at the customer’s site. For more information about the Reliance visit the article webpage.

Reliance™ Autosampler

Spark System Solutions B.V.

Spark Holland has been operating at the forefront of autosampler design for 25 years. Spark’s Reliance™ autosampler is the first true bio-injector, and is preferred by customers for 4 simple reasons: -   Lowest possible carry-over-   High-speed wash cycles-   Low maintenance-   Conditioned sample storage The Reliance™ autosampler complies with GLP requirements and can be coupled with most LC-MS systems. It runs fully integrated with Analyst™ MS software, provides complete traceability, electronic signature authorization and stores the autosampler method in the data file. Integrated in the Symbiosis™ System or operating as a stand-alone unit the Reliance™ autosampler will be a leap forward for the high throughput bio-analytical LC-MS/MS lab.

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AutosamplersAutosamplers are automated sample loaders used with a number of separation and analytical techniques. HPLC autosamplers use automated partial or full-loop injection systems and place samples in vials or microplates for analysis. Autosamplers can also be used for elemental analysis. Features to consider include repeatability, syringe capacity, footprint and temperature controls.  UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Solid-Phase ExtractionSolid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Spark Holland’s Reliance™ autosampler proves to be a true bio-injector