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Spark Holland Announces Fast, Sensitive, Acetonitrile Free Vitamin D Assay

Spark System Solutions B.V.
27 May 2009
Emily Marquez-Vega
Publishing / Media

Product news

A new method for low level determination of the 25-mono-hydroxy-metabolites of vitamin D2 and D3 in plasma has been developed by Spark Holland. The assay is completely acetonitrile free avoiding problems associated with the current acetonitrile shortage.

Quantitation levels of around 1 ng/mL are reported using the Symbiosis™ system for HPLC with integrated Solid Phase Extraction linked to an Applied Biosystems API 4000 Mass Spectrometer. The assay involves almost no manual handling: plasma samples are diluted with a newly developed protein disrupting buffer which also contains the internal standard. Samples are then placed in the autosampler for direct injection into the SPE-LC-MS/MS system. The method shows excellent linearity, precision and carry-over and runs in only four minutes, including SPE.

Low levels of vitamin D have been associated with osteoporosis, diabetes, hypertension and cancer. The new acetonitrile free vitamin D assay is therefore likely to be of significant benefit, in terms of speed, cost effectiveness and convenience to clinical and clinical research laboratory environments.

An innovative development from Spark, the vitamin D assay is the latest example of the high assay quality and sensitivity achieved by dedicated on-line SPE-LC-MS/MS systems. High resolution SPE cartridges and precise SPE process control enable highly efficient sample preparation and concentration. Combined with direct elution into LC-MS/MS systems, high assay performance is achieved as well as maximum assay automation.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Solid-Phase ExtractionSolid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Spark Holland Announces Fast, Sensitive, Acetonitrile Free Vitamin D Assay