NewsLife Sciences
Source BioScience Granted Exclusive Worldwide Licence by the Max Planck Institute to Commercialize the <em>C. elegans</em> and <em>Drosophila melanogaster</em> TransgeneOme Collections
10 Dec 2015
Lois Manton-O'Byrne, PhD
Executive Editor
Industry news
The TransgenOme collections, developed by Dr Mihail Sarov at the Max Planck Institute of Molecular Cell Biology and Genetics, offer a novel opportunity for the exploration of protein function in Caenorhabditis elegans and Drosophila melanogaster.
The collection enhances the Source BioScience portfolio of genome-wide clone resources for use in this important model organism. In addition, the library offers unique applications to complement a wide scope of research. These include:
C. elegans TransgeneOme collection applications exclusively from Source BioScience:
- GFP-tag allows visualisation of protein localisation and expression levels in vivo in many cell types and of all life stages using live fluorescence microscopy or other methods for high-resolution in vivo imaging
- Affinity tags can be used for established methods of protein purification coupled with mass spectrometry for protein-protein interaction analysis
- Approach to map protein binding sites on DNA by tag-based chromatin immunoprecipitation, coupled with NGS (ChIP-Seq) - without the need to develop highly-specific antibodies
Drosophila TransgeneOme collection applications exclusively from Source BioScience:
- expression of tagged proteins under native control - enabling various in vivo applications
- systematic investigation of expression patterns and protein localisation at all developmental stages in different tissues, cell types and subcellular compartments
- identification of interaction partners by purification of protein complexes and subsequent mass spectrometry
- assessment of the extent and functional role of translational control of protein expression by comparing spatial expression data of mRNA with that of proteins
These resources are available on GenomeCUBE®, the interactive proprietary database, search engine and e-commerce tool and can be located by entering GenePair IDs, FlyBase IDs or gene symbols.
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