Smaller Diameter Monolithic HPLC Column Enhances High-Throughput Drug Metabolism Assays
2 Apr 2008Product news
Phenomenex Inc., a global leader in the manufacture of separation science consumables, announces the addition of a new 50 x 2.0 mm ID column to its Onyx monolith line. The 30% smaller diameter column requires less sample volume and reduces solvent consumption. The Onyx columns, which contain solid rods of fused silica, produce results at high flow rates with low backpressure, reducing run times over conventional columns. The permeable media separates viscous biological samples that clog traditional columns, making Onyx ideal for drug metabolism studies in pharmaceutical companies and contract labs.
For fast gradient conditions, flow rates on the Onyx 2.0 mm ID can be increased during re-equilibration without backpressure constraints, significantly reducing overall sample cycle times. The increased speed makes the columns ideal for high-throughput applications. Onyx columns are also available in 3, 4.6 and 10 mm diameters.
"As research companies move toward optimizing their chromatographic processes, Onyx provides a strong platform for faster sample throughput," explained Jason Lam, brand manager for Phenomenex. "With these new 2mm diameter columns, chromatographers can work with low sample concentrations while maintaining high sensitivity and speed - a benefit for fast LC-MS gradient methods."
Phenomenex is a global technology leader committed to developing novel separation, purification and analytical chemistry solutions for the life sciences and related industries. Phenomenex's core technologies include products for liquid chromatography, gas chromatography, sample preparation, bulk purification chromatographic media, and chromatography accessories and equipment.
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Onyx Monolithic LC Columns
Phenomenex IncAcheive Faster HPLCOnyx monolithic silica-based HPLC columns are designed for high-throughput analysis and the direct injection of dirty or viscous samples. Reduce run times with extremely high flow rates (up to 9 mL/min) "Dilute-and-Shoot” dirty biological samples Scalable from capillary to semi-prep column dimensions Onyx columns deliver high efficiency separations and can be coupled together in series to produce the extreme plate counts needed to separate very complex mixtures.Dilute & Shoot Complex mixtures coupled with dirty matrices, such as serum samples encountered in the pharmaceutical/biotechnology industry, are quite difficult and time-consuming to analyze. With Onyx however, you have the option to dilute-and-shoot your sample directly onto the column because it won't clog due to the macroporous system or cause high backpressures. Flow Rate Flexibility Another unique feature is the ability to use flow rate as parameter for optimizing separations. Onyx columns are responsive to changes in flow rate and can be changed mid run to reduce total run time when certain compounds elute much later. This type of flow rate flexibility gives many options for optimization.