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Single-Step, Single-Use Depletion of More than 99% of the Abundant Proteins

BAC B.V.
20 Sept 2010
Sarah Sarah
Marketing / Sales

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BAC launches CaptureSelect® HumanPlasma14 for highly specific, reproducible depletion of the 14 most abundant proteins from human blood plasma or serum. HumanPlasma14 is designed for single-step, single-use depletion of more than 99% of the abundant proteins. The resulting sample can then be used for further downstream applications including gel electrophoresis and mass spectrometry.

HumanPlasma14 is the first product from BAC’s CaptureSelect Proteomics Toolbox, which consists of a mixture of CaptureSelect ligands. CaptureSelect ligands are single-domain monospecific antibodies uniquely immobilized on high-capacity agarose-based beads, which have been proven in laboratory and large scale industrial applications and can be custom-combined for specific protein depletion needs. The slurry can be pre-packed into columns or used in custom-made formats.

“The single-use format of HumanPlasma14 avoids the common problems associated with column recycling, such as clogging, bead regeneration and potential contamination of precious samples”, noted Prof. Marina Hincapie of Northeastern University. “Being able to support a loading capacity of 20-30 ul/ml means that there is less sample dilution and we experience better recovery and reproducibility compared to competitive products.”

Laurens Sierkstra, CEO of BAC said: “HumanPlasma14 is the first product from our Proteomics Toolbox that utilises the unique properties of CaptureSelect technology. By producing highly specific ligands, we are extending our product range to include affordable solutions for protein depletion. Researchers will for the first time benefit from a single-use product that delivers consistent and reproducible results.”

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.

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Single-Step, Single-Use Depletion of More than 99% of the Abundant Proteins