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Simplifying the Transition from Intercalating Dyes to 5’ Nuclease Assays

Integrated DNA Technologies Inc.
24 Jan 2012

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Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, has introduced genome-wide PrimeTime® qPCR Primers, ideal for use with SYBR Green, EvaGreen, and other intercalating dyes in qPCR assays. The PrimeTime qPCR Primers are the same primer pairs used in the well known PrimeTime qPCR Assays, selected to detect and quantify gene expression in human, mouse, and rat transcriptomes. The shipped product consists of a pair of pre-mixed primers, normalized to 5 nmoles, supplying researchers with enough primer for 500 20-µL qPCR reactions with an efficiency of more than 90%.

Pre-designed and synthesised at the time of order, each set of primers incorporates the most up-to-date sequence information, along with accurate melting temperature (Tm) and secondary structure predictions. Each is BLAST searched to eliminate off-target effects and to avoid the impact of single nucleotide polymorphisms (SNPs).

Stephen Gunstream, VP Marketing notes, “With the emergence of next gen sequencing, the number of newly identified SNPs and transcript variations is growing exponentially. Because all of our PrimeTime assays are designed using the latest versions of the RefSeq and SNP databases, we’re able to avoid areas that could lead to inaccurate results. Inventoried assays often use older sequence information with primers placed on top of newly discovered SNPs, which can cause significant errors in measurement of gene expression fold change. Our assays are designed in advance but checked against current sequence and synthesized once an order is placed.”

Since the same primer pairs are used across the PrimeTime qPCR Primers and PrimeTime qPCR Assays, the transition from discovery to screening is easy. When you need to achieve greater specificity and sensitivity for screening applications, you can simply select the probe-based assays.

PrimeTime qPCR Primers are compatible with leading master mixes and have been successfully used under normal and fast cycling parameters. Primer sequence information is provided upon order and primer pairs are shipped within 2 to 3 business days. It is easy to order PrimeTime qPCR Primers through the dedicated PrimeTime qPCR Assay selection tool, or RealTime PCR tool.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Chem / BioinformaticsCheminformatics and bioinformatics are computational techniques used in chemistry and biology, respectively, for data acquisition, processing and storage. Cheminformatics focuses on compound information, whereas bioinformatics is mainly applied to analysis and modeling of genomics, genetic and sequencing information. Hardware and software is available for data acquisition, analysis, management and storage.SNPs TechnologySingle nucleotide polymorphisms (SNPs) are individual base variations in a DNA sequence. SNPs are used in research to study predispositions to disease and drug discovery. Products for studying single nucleotide polymorphisms include SNP arrays and detection systems to detect polymorphisms, SNP typing systems for genotyping DNA, and PCR to amplify specific SNPs.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.SYBR GreenqPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.Evagreen

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