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Sigma® Life Science Enhances Oligoarchitect™ Online Qpcr Tool

Sigma® Life Science
23 Oct 2011

Product news

Sigma-Aldrich® has announced that Sigma Life Science, the innovative biological products and services research business, has released a new version of OligoArchitect™, the comprehensive, easy-to-use online tool for automated design of primers and probes for quantitative real-time PCR assays. OligoArchitect is a free, robust design tool that complements Sigma Life Science’s portfolio of qPCR probes, reagents, and oligonucleotide services.

Powered by the industry standard Beacon Designer™ platform from PREMIER Biosoft, OligoArchitect simplifies compliance with Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines with new features, such as primer and probe homology searching, template secondary structure analysis, and by providing the ability to design across exon splice sites. Additional features include automatic retrieval of template sequences, visualization of the template with positional display of regions of interest, design quality ratings, and tools for alternative and multiplex designs.

“OligoArchitect was designed to empower both qPCR novices and experts to quickly design sophisticated and MIQE-compliant primers and probes without having to shuffle data between multiple tools,” said Tom Russell, Product Manager, Sigma Life Science. “Novices especially will appreciate OligoArchitect’s straightforward, learn-as-one-works design. If additional information is required, a guide to all parameters as well as consultative technical support are available.”

OligoArchitect Online currently supports detection chemistries for both SYBR® Green I primers and dual-labeled probes, making it ideally suited to key applications in traditional PCR, endpoint genotyping, gene expression analysis, genomic copy number determination, allele discrimination, SNP detection, haplotyping, and multiplexing.

For more information about Sigma Life Science’s custom qPCR probes, please visit the company artcile page.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.SYBR GreenMIQE

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Sigma® Life Science Enhances Oligoarchitect™ Online Qpcr Tool