Sigma-Aldrich Launches SpyLine™ Poly A+ Capture Kit

23 Jan 2006

Product news

Sigma-Aldrich (Nasdaq: SIAL), a leading Life Science and High Technology company, is pleased to introduce the SpyLine Poly A+ Capture Kit (product codes: SPY1 and SPY4).

SpyLine is the newest addition to Sigma's RNA product offerings and is an excellent tool for rapidly identifying the knockdown of gene expression for functional profiling of biological processes. "The SpyLine Poly A+ Capture Kit simplifies knockdown measurements and gene expression analysis by allowing capture and amplification of mRNA in a single plate," said Keith Jolliff, Strategic Marketing Director at Sigma- Aldrich. "We are very excited about the sensitivity and reproducibility of the kit, making it an excellent addition to our RNA product offering." The kit features the SpyLine Poly A+ Capture Plate, a 96-well PCR plate coated with oligo(dT) for selective capture of poly A+ mRNA from crude mammalian cell lysates. Reagents can be added directly to this capture plate for subsequent RT-PCR or real-time quantitative RT-PCR cycling and analysis. In addition, the 96-well format is suitable for automation. High throughput protocols are available for the Biomek® FX Workstation.

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Sigma-Aldrich Launches SpyLine™ Poly A+ Capture Kit