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Sigma-Aldrich Announces mirPremier™ microRNA Isolation Kit for miRNA Purification

Merck
1 Nov 2007
Diane Gaige
Marketing / Sales

Product news

Sigma-Aldrich™ is pleased to introduce mirPremier™ microRNA Isolation Kit for purification of miRNA from diverse biological sources. The kit provides a rapid and efficient method for purifying and enriching miRNA along with other small RNAs, allowing researchers to obtain high-quality miRNA directly from cells or tissues as rapidly and simply as a total RNA preparation.

Using mirPremier microRNA Isolation Kit, researchers can purify miRNA/small RNA from diverse biological materials, including mammalian cell cultures, animal tissues, microbial cultures and plant tissues. mirPremier also allows researchers to purify miRNA/small RNA with high yield from difficult-to-extract tissues, such as grape leaf.

The mirPremier microRNA Isolation Kit can be used for isolating total RNA if messenger RNA or other large RNAs are of interest. In addition, mirPremier microRNA Isolation Kit provides an easy-to-use protocol, allowing scientists to purify miRNA within 30 minutes.

"Timely extraction of miRNA from cells or tissues is critical for downstream applications. The mirPremier microRNA Isolation Kit provides a robust protocol for quick extraction of miRNA, making the mirPremier an ideal solution for rapid preparation of miRNA," said Dr. David Smoller, President of the Sigma-Aldrich Research Biotech Business Unit. "We're very pleased to add this new product to Sigma-Aldrich's growing MISSION® RNAi portfolio, as it further supports our strong commitment to the rapidly developing area of RNAi."

"Isolation of miRNA and other small RNAs using the mirPremier microRNA Isolation Kit yields a highly pure and concentrated sample, using a simplified and streamlined protocol without the need of hazardous organic solvents such as phenol and chloroform," said Tim Fleming, Director, Global Commercial Marketing - Life Science for Sigma-Aldrich. "Unlike other miRNA purification methods, mirPremier provided highly purified miRNA samples with no detectable large RNA."

The mirPremier microRNA Isolation Kit complements the already robust MISSION RNAi product portfolio, which includes a broad choice of MISSION siRNA and shRNA products and services such as libraries, mRNA detection reagents, antibodies and AQUA peptides for protein level detection as well as an entire workflow of supporting reagents (cell culture, cell biology compounds and assays, transfection reagents, and much more). For more information, visit the article webpage.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.

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