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Shimadzu Scientific Instruments Announces the Release of the New PPSQ-51A Single Reactor and PPSQ-53A Triple Reactor Protein Sequencers

Shimadzu Corporation

Shimadzu’s new protein sequencers offer enhanced sensitivity and compliance with FDA

10 Mar 2016
Alex Waite
Editorial Assistant

Product news

PPSQ-51A/53A systems use Edman degradation to cleave amino acids, one at a time, from the N-terminals of proteins, then derivatize them to PTH amino acids, and determine the sequence of amino acids using HPLC.

The new PPSQ-51A/53A protein sequencers employ Shimadzu’s SPD-M30A photodiode array detector for higher sensitivity. The detector features a capillary cell that is 8.5 times longer than standard cells, which increases sensitivity and enables the study of longer protein sequences.

PPSQ series protein sequencers separate PTH-amino acids isocratically. Isocratic sequence analysis provides more stable retention times. That means peaks detected in previous cycles can be cancelled using chromatogram subtraction, making it easier for users to identify the correct amino acid. Performing PTH-amino acid analysis in isocratic mode also enables laboratories to reduce liquid waste and running costs through mobile phase recycling.

New PPSQ software can be configured to meet a laboratory’s needs whether it is regulated, research and development, or academic. The software enables compliance with FDA 21 CFR Part 11 guidelines with regard to security, user management, and audit trail requirements. Easy-to-use data analysis functions simplify operation, data processing and reporting. These functions allow the reprocessing of chromatograms, the overlaying of multiple chromatograms, chromatogram subtraction, and performing automatic estimation of amino acid sequences. Additionally, the PPSQ-51A/53A sequencers provide customized reports and yield graph display for a quick and comprehensive view of data.

Customers with previous models, can upgrade their existing systems to have the same sensitivity and software as the PPSQ-51A/53A instruments. Upgrade kits are available in both standard editions for high levels of flexibility as well as database and client server editions that enable 21 CFR Part 11 compliance.

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Sample ManagementSample management systems include sample storage devices such as freezers and plate storers, sample environment enclosures and sample organization, retrieval and sorter systems. Useful system features include high-throughput, automation, robotic arms, automated liquid handling and associated database systems. Accessories in sample management include barcode scanners, heat sealers and tubes.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. 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Useful features of robotic instruments include speed, reproducibility, barcode readers, software and automation.Robotic WorkstationsRobotic workstations are automated platforms enabling the user to perform high-throughput, multi-application processes, from sample preparation tasks such as PCR set-up, DNA purification and NGS library preparation, to integrated sample prep and analysis workstations. Automation makes processes consistent, fast, precise and fully walk-away. Find the best robotic workstations in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Software PlatformsSoftware platforms are useful for various stages of laboratory experiments from data collection to data storage and processing. 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Shimadzu Scientific Instruments Announces the Release of the New PPSQ-51A Single Reactor and PPSQ-53A Triple Reactor Protein Sequencers