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Selectively Recover Phosphopeptides for Mass Spectrometric Analysis Rapidly
24 Jun 2010Sarah Sarah
Marketing / Sales
Product news
The Thermo Scientific Pierce Fe-NTA Phosphopeptide Enrichment Kit can process protein digests and strong cation exchange fractions to selectively recover phosphopeptides for mass spectrometric analysis in less than 30 minutes.
The Pierce Fe-NTA Phosphopeptide Enrichment Kit supplies high-capacity Fe-NTA spin columns and optimized buffer for analyzing 30 protein digests. The phosphopeptide-specific resin in each spin column will sufficiently enrich up to 150 micrograms of phosphopeptides. The Pierce Fe-NTA Spin Columns complement other Thermo Scientific lysis, reduction, alkylation and digestion reagents and graphite spin columns to provide a complete workflow for phosphopeptide enrichment.
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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer.
ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.