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Selective detection of bioactive compounds

CAMAG
2 Mar 2007

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Effect-directed analysis combining HPTLC with bioassays is attracting growing interest in connection with fast toxicity screening for environmental and food-related applications, in the risk assessment and monitoring of drinking water, wastewater and natural attenuation processes, and in the identification of biological activity in natural product extracts.

This example shows the biological activity of berberine containing drugs by HPTLC-bioluminescence screening. Berberine alkaloids are considered to be the active ingredients of many medicinal plants and their biological activity is revealed by the Bioluminex™ assay.

Berberine alkaloids can be easily detected by their green to yellow fluorescence under UV 366 nm. The blue arrow indicates an additional compound which can not be seen under UV 366 nm

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ADME-ToxicologyADME-toxicology (ADME-Tox) studies are used in pharmacology and pharmacokinetics to assess the activity/toxicity of drugs <i>in vivo</i> or <i>in vitro</i>. Find bioassays for absorption, distribution, metabolism, and excretion of drug molecules including cytotoxicity, transporter/permeability, metabolism and activity assays as well as hepatocytes and cell lines for ADME. Find the best ADME-toxicology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Environmental Monitoring and TestingEnvironmental monitoring and testing uses handheld portable analyzers, kits, spectrometers or chromatography systems for air, water, soil, food and other sample testing. Useful features of analyzers such as BOD and COD include portability, easy calibration, automation and sensitivity.  Environmental test kits for pH, water, moisture, etc, should be accurate, sensitive, reliable, fast and easy to use.Thin Layer ChromatographyThin-layer chromatography (TLC), also called planar chromatography, is an inexpensive and simple liquid chromatographic technique used to separate and identify small amounts of compound in a mixture, monitor progress of a reaction or determine purity of substance. TLC plates come in multiple sizes with common adsorbent layers being silica, aluminum oxide or cellulose and high-performance (HP-TLC) plates are also available for increased sensitivity. Other equipment needed includes TLC developing chambers, applicators, TLC sprayers and visualizing devices, such as a viewing cabinet and UV lamp. Find the best TLC products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Selective detection of bioactive compounds