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See the Surface: Accessible TIRF Microscopy Solutions from Olympus

EVIDENT
5 Jul 2015
Sarah Thomas
Associate Editor

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Representing the latest in total internal reflection fluorescence (TIRF) microscopy technology, the expanded Olympus cellTIRF family of illuminators and accessories presents a solution for every application.

Create the ideal TIRFM system

Allowing users to optimize their setup, the new economic one-line illuminator (cellTIRF-1L) complements the high-end, 4- and 2-line (cellTIRF MITICO 4L/2L) illuminators. The new illuminator can also be used with 3rd party lasers and enables multi-fluorescence samples to be sequentially imaged at the same penetration depth. The range supports both precise imaging and easy operation – with features including advanced optics, integrated laser safety systems and high-speed control of penetration depth and laser lines via the Olympus Real-Time Controller. The high-end systems provide ultra-sensitive truly simultaneous multi-color TIRF microscopy, with up to 4 laser lines and independent beam paths. In addition, the functions of the TIRF illuminator are seamlessly integrated into Olympus cellSens software allowing fast and flexible visualization of intracellular molecular events with exceptional speed, sensitivity and resolution.

Outstanding optics

The cellTIRF illuminators are complemented by a large portfolio of specially designed TIRF objectives, including the world’s highest numerical aperture (NA – 1.7) APO 100X OHR, allowing extreme TIRF angles, and the UAPON 150xO TIRF, which combines extreme magnification and high numerical aperture (1.45) with a correction collar and DIC compatibility. Through outstanding optics matched with high-end motorized TIRF devices, Olympus offers a system enabling easy access to super-resolution.

One frame, infinite possibilities

The range is fully compatible with the innovative modular IX3 series of inverted microscope frames. These versatile ‘open source’ frames provide the chance to build bespoke systems with interchangeable optical modules and accessories, thanks to an accessible infinite light path. Modules include the Z-drift compensation system, enabling constant focusing even at high magnifications with dedicated TIRF objectives. Perfect for time-lapse experiments, this also complements the workflow-oriented cellVivo incubation system.

Features

  • cellTIRF-1L module with motorized TIRF angle control offers cost-effective flexibility
  • cellTIRF MITICO 4L/2L high-end, 4- and 2-line illuminators
  • Expansive portfolio of specially designed Olympus TIRF objectives
  • Precise and intuitive control via Olympus cellSens software
  • Compatible with IX3 inverted microscope frames for ultimate flexibility

Applications

  • Live cell imaging
  • Total Internal Reflection Fluorescence (TIRF) Microscopy
  • Cell surface studies
  • Super-resolution microscopy

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Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell ImagingCell imaging can be achieved using a number of techniques including confocal microscopy, transmission electron microscopy, atomic force microscopy, and light sheet microscopy.Fluorescence MicroscopyFluorescence microscopy has become an essential tool in biology, as well as in materials science. The application of many fluorochromes has made it possible to identify cells and sub-microscopic cellular components with a high degree of specificity. Using multiple fluorescence labels, different probes can simultaneously identify several target molecules.Cell AnalysisThe analysis of cells allows researchers to understand the factors which contribute to cell health and function. These influencing processes can then be predicted and altered, leading to the development of medication and disease treatments.Live Cell ImagingLive cell imaging is the study of living cells using microscopes and high-content imaging systems. This technique provides in-depth insight into fast and complex biological processes, by allowing dynamic imaging of living cells instead of acquiring an individual image at a single point in time.MicroscopyMicroscopy is a technique used to observe small objects in detail, from cells to materials, using light or electron microscopes. It enables researchers to examine structures with high resolution, aiding in fields such as biology, medicine, and materials science. With advanced microscopy techniques, scientists can gain insights into cellular processes, tissue structures, and material properties. Explore the best microscopy solutions in our peer-reviewed product directory, compare products, read customer reviews, and get pricing directly from manufacturers.TIRF MicroscopySuper Resolution Microscopy

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See the Surface: Accessible TIRF Microscopy Solutions from Olympus