Screening Unit Use Tecan's Freedom EVO® for Molecular Pharmacology

4 Jul 2010
Sarah Sarah
Marketing / Sales

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Tecan’s Freedom EVO® workstation is being used by the Leibniz-Institut für Molekulare Pharmakologie in Berlin, Germany for systematic high throughput screening of bioactive small molecule libraries, looking for potential research tools and targets for drug development.

Dr Jens Peter von Kries, Head of the Institute’s Screening Unit, explained: “The Screening Unit has a small team of staff performing library screens of around 20,000 compounds a day, and this high throughput is partly thanks to the flexibility of our Freedom EVO workstations. Many of our projects use either high content screening with automated microscopes or genome-wide RNA interference (RNAi) studies, and we have a Freedom EVO system configured for each of these methods, as well as a platform equipped with a REMP Small Size Store™ for management of the ChemBioNet screening library.”

“Each workstation has been collaboratively designed with the Tecan Integration Group (TIG) to create a robust system that is capable of high throughput analysis, giving consistent results even with long term use. The approval procedure is central to this process, with both factory and site acceptance tests to guarantee that the platform exactly matches our needs. Tecan is very willing to support us with development of the platforms as new technologies become available, and we enjoy a very good working relationship with the company’s automation engineers, receiving immediate expert support for all our projects. Tecan has an exceptional culture of customer service, and our work here at the FMP undoubtedly benefits from this close partnership.”

Tecan Freedom EVO® Workstation

Tecan

The Freedom EVO platform offers fully automated liquid handling and robotics for life sciences and diagnostics. This flexible system offers virtually unlimited options, which can be tailored to provide individual solutions.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Screening Unit Use Tecan's Freedom EVO® for Molecular Pharmacology