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Scientists Rate Secondary Antibodies from Jackson ImmunoResearch Laboratories Inc.

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Find out what the experts really think

23 Oct 2017
Lynsey Forsyth
Post Doc / Research Fellow

Editorial article

Image: Shutterstock/Antibodies_123

Jackson ImmunoResearch Laboratories, Inc. specializes in the production and conjugation of affinity-purified secondary antibodies and purified immunoglobulins. Their products are sold primarily to scientists in universities and research institutes throughout the world that are conducting research in the plant, animal and biomedical sciences.

Read on to discover what scientists are saying about Jackson ImmunoResearch antibodies and how they could enhance your research.


Beth Viviano at Washington University uses affinity-purified secondary antibodies for immunohistochemistry.


I have used these secondary antibodies for years and they always work great.

 


Beth Viviano

Washington University

Peter Zhang at the University of Michigan uses light chain specific secondary antibodies for western blotting.


This Ab is easy to use and we can get high quality and reproducible results using it. It's very specific for the light-chain. My rating for this Ab is 5 star.

 


Peter Zhang

University of Michigan

Mario Valentino at the University of Malta uses affinity-purified secondary antibodies for immunocytochemistry.


Affordability, specificity and high signal coupled to long shelf life make these secondary antibodies our first line of choice in ongoing research in our lab. The company is also very helpful and supportive.

 


Mario Valentino

University of Malta

Kristian Jensen at the University of Copenhagen uses biotinylated antibodies with conjugated streptavidin to trace biocytin injected into neurons.


Reliable and specific stain each time.

Kristian Jensen  University of Copenhagen


Do you use Jackson ImmunoResearch’s antibodies? Share your expertise and write your own review today.

Learn more about Jackson ImmunoResearch Laboratories Inc. in this exclusive interview and on their website.

Visit the SelectScience antibody directory to read more impartial reviews, and find the right antibody for your research.

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Streptavidin and Conjugates

Jackson ImmunoResearch Laboratories Inc.

Using biotinylated secondary antibodies along with conjugated streptavidin is a robust means of signal amplification. Conjugates of streptavidin are recommended for use with Biotin-SP-conjugated affinity-purified secondary antibodies and Biotin-SP-conjugated ChromPure proteins. We offer a comprehensive list of fluorophores and enzymes conjugated to streptavidin for use in enzyme immunoassays, immunohistochemistry, flow cytometry, in situ hybridization, and immunoblotting procedures.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Engineered AntibodiesEngineered antibodies are modified to enhance specificity, stability, and functionality for therapeutic and diagnostic applications. These antibodies are central to immunotherapy and biomarker detection. Compare engineered antibody solutions in our directory to find top products and reviews.

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