Save Hours of Dry-Down Time for Your Prep HPLC Fractions

The speed of purification and level of purity obtained with Preparative High Performance Liquid Chromatography (Prep HPLC) has increased dramatically over the last 20 years such that it is no longer a bottleneck in the pharmaceutical development cycle. However, delays have shifted downstream to the time taken to recover the purified product.

Whilst a prep HPLC run can be completed in less than 15 minutes, it can take 8-24 hours to recover the purified compound, dry it, reconstitute it in the desired solvent and format it into a compound library. This is due to the usually high water composition of the collected fraction, the potential need to change the product structure and remove compounds such as TFA, plus re-formatting the compound into another solvent or structural form.

Now, with Anachem's new FT-LC system this bottleneck is virtually eliminated. This system has been developed around the industry standard Gilson Prep HPLC platform, a unique specifically designed trapping column technology, FlowTrap™ from Varian Inc. and uses application specific software.

The FT-LC system can dramatically reduce the water content of fractions collected from the prep HPLC system, from 88% to less than 1% in under 15 minutes. Included in the process is a step to automatically convert compounds from acid to base form. Recoveries have been consistent with findings in prep HPLC of > 90% for the whole process and it has already been used and verified by several major pharmaceutical companies.