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Sage Science Launches Proteomics Early Access Program for SageELF Instrument

Sage Science
31 Mar 2015
Sarah Thomas
Associate Editor

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Sage Science, a developer of products for improving sample preparation processes in life science applications, today announced the launch of a proteomics early access program for its SageELF technology. SageELF is a protein sample prep tool useful for biomarker discovery, protein profiling, shotgun proteomics, quality control of biotherapeutics, and more.

With this early access program, Sage Science expands the applications of the SageELF instrument from whole-sample DNA fractionation to protein fractionation for proteomic workflows. The SageELF generates 12 contiguous fractions from a protein sample in a fully automated process that is more sensitive and reproducible than manual 1D gels. The tool can be used to reduce sample complexity, enrich for rare proteins, and improve recovery of all proteins in a sample.

“SageELF has been very well received by users in the genomics community, and now we are eager to see what users in the proteomics community will accomplish with it,” said Todd Barbera, Chief Executive Officer of Sage Science. “We have already seen remarkable results with antibody experiments and biomarker discovery studies, and we anticipate that our early access researchers will demonstrate many other creative uses and high-quality data with this unique technology.”

The SageELF, short for Electrophoretic Lateral Fractionator, automatically electrophoreses samples and elutes proteins from the entire gel lane into 12 elution modules. Users simply load their sample, start the run, and pipette out the fractions later. Unlike in-gel digestion, in which some peptides never leave the gel, the whole protein sample is electrophoresed into the elution modules.

Scientists interested in joining the SageELF early access program can visit http://www.sagescience.com/elf-protein/ for more information. The SageELF instrument will also be on display at the upcoming annual meeting of the Association of Biomolecular Resource Facilities (ABRF) in booth #323 and at the Experimental Biology conference in booth #1065.

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SageELF Sample Fractionator for DNA and Protein

Sage Science

The SageELF is an automated, whole-sample fractionation system for NGS sample prep. SageELF, a whole-sample fractionation tool, is now shipping from Sage Science, developer of the Pippin automated DNA size selection system. The SageELF (short for Electrophoretic Lateral Fractionator) generates 12 contiguous fractions from a whole DNA sample, allowing scientists to generate libraries with multiple insert sizes from the same sample, which has applications in genome sequencing, structural variant detection, and identification of rare splice variants. This sample prep instrument will also be especially useful for preserving precious samples. Users simply load their sample, start the run, and pipette out the fractions later. Unlike in-gel digestion, in which some DNA fragments never leave the gel, the whole DNA sample is electrophoresed into the elution modules.Sage Science targets labor-intensive steps in the sample prep process where automation can offer improved precision and reproducibility. Precise size selection is critical for optimizing sequencing efficiency, improving genome assemblies, and reducing NGS project costs.Features: Make libraries with multiple insert sizes from the same sample Ideal for rare or precious samples Automated for precise, reproducible results Applications: Genome sequencing Structural variant detection Rare splice variant detection

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Sage Science Launches Proteomics Early Access Program for SageELF Instrument