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Roche RT-PCR technology used for TIB MOLBIOL´s H1N1 test

4 May 2009
Samantha Rosoman
Campaign Coordinator

Product news

TIB MOLBIOL, the Berlin-based cooperation partner of Roche Applied Science announced today the development of a LightMix test for the new variant of the Influenza A H1N1 virus, to be launched initially in Europe and Asia. This test has been optimized for Roche LightCycler® 480 II, 2.0, and 1.2 systems and selectively identifies the new Influenza A H1N1 virus.

“We are proud of our contribution in the battle against a potential pandemic. The new test proved its suitability this week, after identifying the virus in sample materials from Mexico,” said Olfert Landt, TIB Molbiol.

Manfred Baier, Head of Roche Applied Science, adds: “Roche is committed to supporting governments and institutions worldwide in combating this outbreak of influenza, and we are providing scientists, who are working on Influenza A H1N1 virus, with efficient tools for their work.”

Applied Science offers a variety of platforms that can be used to help identify the Influenza A H1N1 virus, including Nucleic Acid Purification, Real Time PCR, Microarrays and Sequencing systems. The company is in close contact with various research institutions and test centers worldwide providing assistance in the detection and characterization of Influenza A H1N1 virus. Basing on these contacts Roche Applied Science is developing another test with improved characteristics, which will be available soon.

Currently, there is no human vaccine on the market that protects against Influenza A H1N1 infection. Standard therapy for the disease includes treatment with antiviral drugs like Tamiflu or Relenza. For more information on Influenza A H1N1 please visit www.who.int.

Since 1990, TIB MOLBIOL has been offering custom synthesis of oligonucleotides to a growing number of customers worldwide. As one of the first suppliers of synthetic DNA on the European market, the company has since set standards of quality and service. Today the core competence lies in the development of Real-Time-PCR assays for life science research, environmental analysis and medical diagnostics.

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Point-of-CarePoint-of-Care Testing (POCT) or Near Patient Testing (NPT) products are available for urine, blood and other clinical chemistry analyses. POCT includes: blood glucose testing, blood gas and electrolytes analysis, rapid coagulation testing (PT / INR), rapid cardiac markers diagnostics, drugs of abuse screening, urine strips testing, pregnancy testing, fecal occult blood analysis, food pathogens screening, hemoglobin diagnostics, infectious disease testing and cholesterol screening.Clinical GeneticsMolecular Genetics covers the analysis of hereditary genetic disease and chromosomal abnormalities. Genetics can be analysed using DNA, RNA, and protein microarrays, PCR, RT PCR and DNA sequencing. Genetic equipment includes genetic workstations, thermal cyclers, cooling blocks and electrophoresis products. Diagnostic kits are used for DNA / RNA extraction and purification.

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Roche RT-PCR technology used for TIB MOLBIOL´s H1N1 test