SelectScience
Join Free
Accelerating Science
NewsLife Sciences

Roche Launches New Tools for RNA Isolation and cDNA Pre-Amplification From FFPET Samples

31 Oct 2012

Product news

Roche has announced the global launch of its new High Pure FFPET RNA Isolation Kit as well as the RealTime ready cDNA Pre-Amp Master & Primer Pools. Formalin-fixed, paraffin-embedded tissue (FFPET) samples from biopsies or micro-dissections are frequently used for retrospective studies, primarily in oncology. They also represent an increasingly important resource for biomarker research and investigating disease progression or drug responsiveness.

The new High Pure FFPET RNA Isolation Kit delivers RNA of excellent integrity and yield – two key factors in ensuring high-quality results in the diversity of downstream applications such as qRT-PCR, microarray analysis and sequencing. For comprehensive gene expression studies, the isolated RNA can be transcribed into cDNA and subsequently pre-amplified with the new RealTime ready cDNA Pre-Amp Master. This kit makes it possible to pre-amplify minute amounts of cDNA with customized primer pools that correspond perfectly to the primers which can be applied in subsequent qPCR analysis. This approach ensures unbiased amplification and a flexible fit to any customer’s genes of interest.

“Our new products provide highly convenient and flexible solutions to researchers who are analyzing gene expression in FFPET samples, while ensuring excellent result quality,” said Gerd Haberhausen, Business Head of qPCR and Sample Preparation Systems. “By offering customized, aligned primer sets alongside our new RNA isolation kit, we are able to provide a complete and reliable workflow to scientists investigating the mRNA expression profiles of complex biological pathways.”

The High Pure FFPET RNA Isolation Kit’s superb performance has been proven by analyzing FFPET samples of different ages and by demonstrating its applicability in biomarker research. It produces powerful signals downstream in qPCR, microarray analysis, and sequencing. RNA from formalin-fixed, paraffin-embedded tissue, macro-dissected and laser-micro-dissected samples, and biopsies can be isolated in just 2.5 hours, including deparaffinization.

Visit the SelectScience Application Note Library to find out more.

Tags

BiosensorsBiosensors are devices used to detect an analyte using biological molecules specific to the analyte coupled to a detector. Biosensor instruments may be photometric, typically using surface plasma resonance (SPR), electrochemical or QCM (quartz crystal microbalance) biosensors. Biosensors should be selective, portable, robust and sensitive and have a fast response time. Systems may be manual or automated and usually have associated software.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Medicinal ChemistryMedicinal chemistry is a broad discipline encompassing the design, identification, synthesis and development of chemicals in drug discovery. It includes a number of techniques covering structural biology, synthetic chemistry and molecular biology. Technologies used in medicinal chemistry include ADME, lab-on-a-chip, high content screening and assay assembly.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Cancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.Gene ExpressionReal-Time PCRReal-time PCR (RT-PCR) is a technique used to amplify and simultaneously quantify nucleic acids in real time. It is a key tool in gene expression analysis, pathogen detection, and diagnostics. RT-PCR allows for the detection of specific RNA or DNA sequences with high sensitivity and specificity. Browse our peer-reviewed product directory to find the best Real-Time PCR systems; compare products, check reviews, and get pricing directly from manufacturers.FFPEFormalin-fixed paraffin-embedded (FFPE) tissues are commonly used in medical research, particularly in histology and cancer studies. The preservation process allows long-term storage of tissue samples for later analysis. Explore FFPE research products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

Related articles

Roche Launches New Tools for RNA Isolation and cDNA Pre-Amplification From FFPET Samples