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Roche and NuGEN Technologies Develop Whole Transcriptome Profiling Solution for 454 Sequencing Systems

4 Apr 2011
bridget bridget
Laboratory Director

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454 Life Sciences, a Roche Company, and NuGEN Technologies, announced today the development of a unique sample preparation method and high-throughput sequencing solution for small and degraded RNA samples. The collaboration leverages 454 Life Sciences’ capabilities to construct sequencing-ready cDNA libraries from RNA samples and NuGEN’s experience and innovation with genomic sample preparation solutions. This collaborative effort provides researchers an end-to-end solution for transcriptome profiling using their most precious sample types.

Rare and difficult to obtain samples present a unique challenge to researchers who must maximize the utility of low abundance sample. Typical cDNA protocols often require up to 20 µg of input material which limits use to samples with large quantities of RNA available. Under the collaborative relationship, the two companies have developed a solution using both NuGEN’s proprietary isothermal linear amplification (SPIA®) products and the GS FLX Titanium cDNA Rapid Library Preparation protocol for the GS Junior and GS FLX Systems.

“NuGEN’s innovative sample preparation solutions provide a unique and powerful tool which will enable researchers to obtain high-quality data from their less abundant samples,” said Elizabeth Hutt, CEO of NuGEN Technologies. “Together with 454 Sequencing Systems, the research community can perform whole transcriptome profiling on samples with as little as 500 pg of total RNA. Being able to collaborate with a leading NGS platform provider such as Roche, enables customers to leverage NuGEN’s linear amplification technology while obtaining long and highly accurate sequence reads.”

“We are sensitive to the challenges many researchers face when working with precious and often irreplaceable samples,” said Todd E. Arnold, Ph.D., Vice President of Development at 454 Life Sciences, a Roche Company. “By combining NuGEN’s amplification technology with 454 Sequencing Systems, we provide a solution that meets these researchers’ needs, while allowing them to leverage the power of 454 Sequencing System’s long read lengths which are critical for both de novo transcriptome assembly and comprehensive identification of splice variants and fusion transcripts.”

For more information on 454 Sequencing Systems, visit the company article page.

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GS Junior System

The power of next-gen sequencing in your hands. The GS Junior System brings the power of 454 Sequencing Systems directly to the laboratory benchtop. Get comprehensive genome coverage with long 400 bp sequencing reads and quickly proceed from DNA to discovery with fast sequencing runs and straightforward data analysis on the attendant computer.GS Junior System Features: Powered by proven technology: Uses GS Junior Titanium chemistry to deliver high-quality, bioinformatics-friendly, long read data Fits in your lab: Small instrument size, along with low entry and operating costs, enables labs with limited budget and infrastructure to take advantage of next gen sequencing Easy to use: Manageable data size allows processing and analysis right on the attendant computer without the need for specialized computing or network infrastructures Point-and-click data analysis: Includes the GS Data Analysis Software for de novo assembly, reference mapping and amplicon variant analysis Applications: Amplicon Sequencing: Targeted sequencing of 10's - 100's of samples and loci Sequence Capture: Targeted sequencing of custom array regions Whole Genome Sequencing: De novo sequencing and resequencing of microbial organisms (bacteria, fungi, viruses) Metagenomics: Characterization of complex environmental samples; pathogen discovery Transcriptome Sequencing: Full-length sequencing and de novo assembly of transcripts

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GS FLX+ System

Roche

Now delivering sequencing reads up to 1,000 bp in length! The GS FLX+ System features the unique combination of long reads, exceptional accuracy and high-throughput, making the system well suited for larger genomic projects. The GS FLX System has been at the heart of key breakthrough genomic discoveries and over 1,000 peer-reviewed publications to date.Advancements in sequencing chemistry, instrumentation and software offer the latest improvements in GS FLX System performance, with read lengths up to 1 kb. Available as an on-site instrument upgrade or new instrument, the GS FLX+ System is designed for use with both the new long-read Sequencing Kit XL+ and existing Sequencing Kit XLR70.GS FLX+ System Features: True capillary sequencing-like read lengths: Continuous development of the GS FLX Titanium chemistry now offers read lengths up to 1 kb Flexible sequencing formats: Broad selection of gaskets and Multiplex Identifiers (MIDs) enables efficient use of sequencing runs Point-and-click data analysis: Included GS Data Analysis Software for de novo assembly, reference mapping and amplicon variant analysis. Applications: Whole Genome Sequencing: De novo sequencing of large, complex organisms or multiple bacterial genomes in a single run Transcriptome Sequencing: Full-length de novo sequencing and assembly of complex organisms Amplicon Sequencing: High-throughput sequencing of 100s to 1,000s of samples and loci Sequence Capture: Targeted resequencing of whole exomes or large capture regions Metagenomics: Characterization of complex environmental samples; pathogen discovery

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