Roche and Hamilton Introduce New Automated DNA Sample Enrichment Platform for GS Junior Sequencing Instruments

28 Feb 2013

Product news

Today Roche and Hamilton Robotics jointly announced the introduction of a new, integrated platform enabling automated DNA sample enrichment for Roche’s 454 GS Junior sequencer. The platform builds on the integration of Roche’s REM e System with Hamilton’s Microlab® NIMBUS NGS workstation, resulting in reduced hands-on time, minimized operational variability and more consistent results.

“This new integrated platform will replace about four hours of laborious manual work,” said Thomas Schinecker, head of 454 Life Sciences “It is a true walkaway solution, offering an economical, convenient, compact enrichment procedure for all our GS Junior customers. We look forward to continuing our collaboration with Hamilton to develop more innovative, automated solutions for our GS Junior System.”

The new platform has been fully tested and validated to automate the emPCR breaking, bead enrichment and sequence primer annealing steps in the GS Junior sample preparation workflow. Dedicated software with a graphical user interface (GUI) provides easy automation setup and simple user control.

“The Hamilton NIMBUS NGS Systems are built to speed the liquid handling process by using advanced robotic pipetting technologies,” said Dr. Jose Carle, Vice President, Hamilton Robotics. “With Roche we’ve intentionally kept the systems easy to use, affordable and smaller than conventional systems, thus putting the power of high-throughput genetic analysis and discovery directly into researchers' hands.”

GS FLX+ System

Roche

Now delivering sequencing reads up to 1,000 bp in length! The GS FLX+ System features the unique combination of long reads, exceptional accuracy and high-throughput, making the system well suited for larger genomic projects. The GS FLX System has been at the heart of key breakthrough genomic discoveries and over 1,000 peer-reviewed publications to date.Advancements in sequencing chemistry, instrumentation and software offer the latest improvements in GS FLX System performance, with read lengths up to 1 kb. Available as an on-site instrument upgrade or new instrument, the GS FLX+ System is designed for use with both the new long-read Sequencing Kit XL+ and existing Sequencing Kit XLR70.GS FLX+ System Features: True capillary sequencing-like read lengths: Continuous development of the GS FLX Titanium chemistry now offers read lengths up to 1 kb Flexible sequencing formats: Broad selection of gaskets and Multiplex Identifiers (MIDs) enables efficient use of sequencing runs Point-and-click data analysis: Included GS Data Analysis Software for de novo assembly, reference mapping and amplicon variant analysis. Applications: Whole Genome Sequencing: De novo sequencing of large, complex organisms or multiple bacterial genomes in a single run Transcriptome Sequencing: Full-length de novo sequencing and assembly of complex organisms Amplicon Sequencing: High-throughput sequencing of 100s to 1,000s of samples and loci Sequence Capture: Targeted resequencing of whole exomes or large capture regions Metagenomics: Characterization of complex environmental samples; pathogen discovery

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GS Junior System

The power of next-gen sequencing in your hands. The GS Junior System brings the power of 454 Sequencing Systems directly to the laboratory benchtop. Get comprehensive genome coverage with long 400 bp sequencing reads and quickly proceed from DNA to discovery with fast sequencing runs and straightforward data analysis on the attendant computer.GS Junior System Features: Powered by proven technology: Uses GS Junior Titanium chemistry to deliver high-quality, bioinformatics-friendly, long read data Fits in your lab: Small instrument size, along with low entry and operating costs, enables labs with limited budget and infrastructure to take advantage of next gen sequencing Easy to use: Manageable data size allows processing and analysis right on the attendant computer without the need for specialized computing or network infrastructures Point-and-click data analysis: Includes the GS Data Analysis Software for de novo assembly, reference mapping and amplicon variant analysis Applications: Amplicon Sequencing: Targeted sequencing of 10's - 100's of samples and loci Sequence Capture: Targeted sequencing of custom array regions Whole Genome Sequencing: De novo sequencing and resequencing of microbial organisms (bacteria, fungi, viruses) Metagenomics: Characterization of complex environmental samples; pathogen discovery Transcriptome Sequencing: Full-length sequencing and de novo assembly of transcripts

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Clinical GeneticsMolecular Genetics covers the analysis of hereditary genetic disease and chromosomal abnormalities. Genetics can be analysed using DNA, RNA, and protein microarrays, PCR, RT PCR and DNA sequencing. Genetic equipment includes genetic workstations, thermal cyclers, cooling blocks and electrophoresis products. Diagnostic kits are used for DNA / RNA extraction and purification.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Target-EnrichmentLiquid Handling WorkstationLiquid handling workstations automate the process of sample preparation, improving accuracy and efficiency. These systems are essential for high-throughput applications in biological, chemical, and pharmaceutical research, reducing human error and increasing reproducibility. Workstations come equipped with robotic arms, pipetting tools, and other accessories to streamline lab workflows. Explore liquid handling workstations in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.PCR AutomationPCR automation describes the process of using a thermocycler to complete the polymerase chain reaction (PCR) process quickly and efficiently.
Roche and Hamilton Introduce New Automated DNA Sample Enrichment Platform for GS Junior Sequencing Instruments