Rigaku Introduces the BioSAXS-1000 AUTO for Biological SAXS within Your Laboratory

15 Feb 2013

Product news

Rigaku Corporation introduces the new BioSAXS-1000 AUTO, a leap forward for experimental biological SAXS (small angle X-ray scattering) workflow for the home lab. The BioSAXS-1000 AUTO combines Rigaku’s proven BioSAXS-1000 system with automatic sample handling, automatic data collection and automatic data analysis for biological solution scattering experiments.

Integral to the new BioSAXS-1000 AUTO is a state of the art SAXS analysis pipeline, called AAP (automatic analysis pipeline), that utilizes automated modules from the world’s most popular and comprehensive ATSAS program suite.

Resulting from collaboration between Rigaku and EMBL scientists in Hamburg, Germany, the automatic ATSAS-based pipeline inherent to AAP automates data processing steps such as profile averaging and buffer subtraction as well as data analysis steps such as Guinier and Porod analyses, P(r) calculation, ab initio shape determination and evaluation.

The end result of AAP is a summary of results for several concentrations of your protein solution, presented in an easy-to-review format with hyperlinks to visualize the data and models. With the BioSAXS-1000 and AAP, Rigaku gives you all the tools you need to perform successful SAXS experiments in the home lab and to make the information obtained from SAXS an integral part of your research flow.

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X-ray CrystallographyX-ray crystallography is an analytical technique used to determine the arrangement of atoms in a crystal. Monochromatic x-rays are produced from a synchrotron or x-ray generator. An x-ray crystallography system uses a detector to measure the x-ray diffraction from the crystal. The information is used to generate a 3D image of the crystal.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.X-Ray Diffraction and SpectroscopyX-Ray diffraction & spectroscopy are used in material characterization to discern the structure and elemental composition of a sample. X-Ray diffractometers (XRD) are superior instruments in elucidating the dimensional atomic structure of crystalline materials, including powders, thin films and single crystals. For large unit cells or ordered macromolecules, consider small-angle X-ray scattering (SAXS). X-ray spectroscopic techniques include X-ray fluorescence (XRF) and X-ray photoelectron spectroscopy (XPS), both providing simple and accurate methods for determining the elemental composition of a material. Energy dispersive (EDXRF) and wavelength dispersive (WDXRF) XRF spectrometers are available, as well as handheld/portable devices. High-resolution, 3D microstructure characterization of materials can be achieved with X-ray microscopes combining sub-micron resolution imaging with 3D computed tomography. Find the best XRD and XRF spectrometers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein CrystallographyProtein crystallization is the process of crystallizing purified proteins for 3D structure analysis by x-ray crystallography. The main methods of protein crystallization include sitting drop, hanging drop and microbatch. It is important to control parameters such as pH, temperature and concentration. Following crystallization, detectors and software are used for data collection and analysis.SAXSStructural Biology
Rigaku Introduces the BioSAXS-1000 AUTO for Biological SAXS within Your Laboratory