Reproducible Isolation of High Quality RNA with INTEGRA’s VIAFLO 384 Electronic Pipette

22 May 2013
Sarah Thomas
Associate Editor

Product news

INTEGRA has released a new video demonstrating a solid phase extraction (SPE) microplate method for reproducible, high throughput isolation of high-quality DNA-free total RNA.

Large scale gene expression screening projects require reliable high-throughput methods to isolate high quality RNA from a large number of samples. To meet this need, a growing number of commercial 96-well format RNA isolation kits are now available. Typically such kits are compatible with commonly used 96-well plate vacuum manifolds enabling the extraction (isolation) procedure to be accelerated.

The video shows how researchers use a VIAFLO 384 handheld electronic pipette in conjunction with vacuum manifold, 96-well solid phase extraction (SPE) microplate and RNA isolation kit to reproducibly extract high quality total RNA from cultured cells and bacteria.

The VIAFLO 384 is a handheld 384-channel electronic pipette that offers all the benefits of increased sample throughput as well as lower sample and reagent use without the expense of having to invest in a robotic liquid handling system. Designed to be used just like a standard handheld pipette the VIAFLO 384 requires no special skills or training to operate it. The VIAFLO 384 features INTEGRA's popular Touch Wheel™ pipette user interface enabling simple and fast programming of a wide range of pipetting modes including repeat dispense, serial dilute and mixing routines.

VIAFLO 384 – Electronic 24, 96 and 384 Channel Handheld Pipette

INTEGRA Biosciences

INTEGRA Biosciences’ VIAFLO 384 increases productivity of sample preparation in microplates and supports 24, 96 and 384 channel pipetting heads. VIAFLO 384 is used like a standard handheld pipette. It is hand-controlled but movements are supported by motors for an effortless workflow and precise targeting of the microplate wells. With a choice of ten interchangeable pipetting heads, the unit quickly adapts to any 24, 96, 384, or even 1536 well pipetting application in a volume range of 0.5 μl to 1250 μl.  VIAFLO 384 benefits from the innovative GripTip system. The special pipette tips, together with the unique tip fitting, form an integral system. It guarantees tips will not fall off during pipetting and provides a reliable sealing of every tip. VIAFLO 384 can be used for sample transfers from reservoir to plate, plate to plate or within plates. VIAFLO 384 Features and Benefits: VIAFLO 384 is used like a handheld pipette and has an intuitive user interface, freeing staff from training time. Pipetting heads can be changed for an optimal instrument adaption to different volume ranges, increasing accuracy and precision. The innovative GripTip system provides a reliable sealing of every tip and guarantees that tips will never fall off, providing safe and reproducible assay preparation. Various pipetting modes are available for increased productivity and application customization.

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Pipettes and DispensersPipettes and dispensers are essential lab equipment used to dispense measured volumes of liquid accurately. Explore a range of manual and electronic pipettes, including single and multichannel, serological and volumetric pipettes, as well as a range of pipette tips. Bottle-top dispensers are also available for simple and fast solvent dispensing. Find the best pipettes & dispensers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNARNA is a nucleic acid that plays a key role in gene expression and protein synthesis. It serves as a messenger between DNA and ribosomes, carrying genetic instructions to produce proteins. Advances in RNA-based therapies, such as mRNA vaccines and gene editing, have revolutionized treatment strategies for genetic disorders and infectious diseases. Explore RNA research tools and therapies in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.Electronic PipetteElectronic pipettes are precision instruments used to transfer measured volumes of liquid in laboratory settings. They offer high accuracy, ease of use, and ergonomic benefits compared to traditional pipettes, reducing the risk of repetitive strain injuries. Electronic pipettes are widely used in molecular biology, chemistry, and diagnostics. Browse our peer-reviewed product directory to find the best electronic pipettes; compare products, check reviews, and get pricing directly from manufacturers.
Reproducible Isolation of High Quality RNA with INTEGRA’s VIAFLO 384 Electronic Pipette