R&D Systems Now Using LI-COR IRDye Infrared Dyes in Antibody Arrays

R&D Systems is now offering Proteome Profiler™ 96 Infrared Antibody Arrays that utilize IRDye® 800CW Streptavidin from LI-COR® Biosciences. These multiplex kits also use a 96-well microplate pre-spotted with up to 16 different capture antibodies and an HRP-conjugated detection antibody to detect the relative phosphorylation of multiple kinases in a single sample of cell lysate. Fluorescent signals are measured using a LI-COR Odyssey® infrared imaging system, such as the Odyssey CLx, Odyssey Sa, or a compatible infrared imaging system.

“Near-infrared fluorescence provides enhanced fluorescence sensitivity and more accurate quantitation,” says Jim Wiley, LI-COR senior strategic marketing manager. “We are happy to offer the best dye technology to both our and R&D Systems’ customers through the Proteome Profiler™ 96 Infrared Antibody Arrays.”

These multiplex kits use an ELISA-based format for the high-throughput analysis of protein kinase phosphorylation and feature an array in each well of a 96-well microplate. These are complete kits that include a pre-spotted 96-well microplate and all necessary reagents, also requiring only a small sample size (5-50 μg of cell lysate).

“We are excited to team up with LI-COR to expand our Proteome Profiler 96 Antibody Array product offerings with kits that are compatible with the LI-COR Odyssey infrared imaging system,” says Greta Wegner, R&D Systems scientist and array group manager.