SelectScience
Join Free
Accelerating Science
NewsLife Sciences

R&D Systems to Launch New Microplate-Based Profiling Kits at ASCB 2009

Bio-Techne
1 Dec 2009
Sarah Sarah
Marketing / Sales

Product news

R&D Systems will present the new Proteome Profiler™ 96 Microplate-based Antibody Array. Utilizing expertise in antibody and ELISA technologies, it aims to make multi-protein detection more efficient than ever. The assay relies on a 96-well microplate that is pre-spotted with a carefully selected panel of capture antibodies in each well. To find out more visit booth 513.

When experimental samples are added to the wells, up to 16 different proteins can be detected simultaneously in a single sample using a two-site sandwich immunoassay. Proteome Profiler 96 Microplate-based Antibody Arrays offer the advantages of multianalyte profiling in a format amenable to high-throughput analysis. They allow data to be collected in approximately 3.5 hours, utilize small sample sizes (50-100 μL), and can identify proteins of interest without having to perform multiple immunoprecipitation/Western blot experiments.

Related products

Request Quote for All Products

Proteome Profiler Antibody Arrays

Bio-Techne

R&D Proteome Profiler Antibody Arrays R&D Proteome Profiler Arrays allow for the measurement of multiple proteins in a single sample. These arrays require no specialized equipment, and eliminate the need for multiple immunoprecipitation/Western blot experiments. R&D Array kits contain buffers, detection antibodies, and membranes spotted in duplicate with capture antibodies carefully selected for their specificity. R&D Arrays include:R&D Systems Human Phospho-RTK Antibody Array (Catalog # ARY001)Detect the relative site-specific phosphorylation levels of 42 different receptor tyrosine kinases(RTKs). R&D Systems Human Phospho-MAPK Antibody Array (Catalog # ARY002) Detect the relative site-specific phosphorylation levels of 19 different intracellular serine/threonine kinases. R&D Systems Human Phospho-Kinase Antibody Array (Catalog # ARY003) Detect the relative phosphorylation levels of 46 intracellular serine/threonine/tyrosine kinases. R&D Systems Human Phospho-Immunoreceptor Antibody Array (Catalog # ARY004) Detect the relative phosphorylation levels of 59 ITAM/ITIM-associated immunoreceptors. R&D Systems Human Cytokine Antibody Array, Panel A (Catalog # ARY005) Detect the relative levels of 36 cytokines, chemokines, and acute phase proteins simultaneously. R&D Systems Mouse Cytokine Antibody Array, Panel A (Catalog # ARY006) Detect the relative levels of 40 cytokines, chemokines, and more simultaneously. R&D Systems Human Angiogenesis Antibody Array (Catalog # ARY007) Detect the relative levels of 55 angiogenesis-related proteins simultaneously. R&D Systems Rat Cytokine Antibody Array (Catalog # ARY008) Detect the relative levels of 29 cytokines, chemokines and more simultaneously. R&D Systems Human Apoptosis Antibody Array (Catalog # ARY009) Detect the relative levels of 35 apoptosis-related proteins simultaneously. R&D Systems Human Pluripotent Stem Cell Antibody Array (Catalog # ARY010) Detect the relative levels of 15 pluripotent stem cell markers simultaneously.

(1)

Links

Bio-TechneCompany website

Tags

Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

Related articles

R&D Systems to Launch New Microplate-Based Profiling Kits at ASCB 2009