R&D Systems Presents New Tools for Profiling the Phosphorylation of Multiple Signaling Molecules at ASCB 2010

R&D Proteome Profiler Human Phospho-Kinase Array Kit - Human Phospho-Kinase Array Kit for the parallel determination of the relative levels of protein phosphorylation. The kit contains 4 sets of 2 membranes - each spotted in duplicate with 46 different kinase antibodies. The R&D Human Phospho-Kinase Array is a rapid, sensitive, and economical tool to simultaneously detect the relative levels of phosphorylation of 46 kinase phosphorylation sites without performing numerous immunoprecipitations and Western blots. Each capture antibody was carefully selected using cellular extracts prepared from cell lines known to express the target protein. R&D Proteome Profiler Human Phospho-Kinase Array Kit Features:• SPECIFIC - Carefully selected panel of 46 capture antibodies gives low cross-reactivity • EFFICIENT - Tests 46 different kinases simultaneously with a small sample size • SENSITIVE - Comparable to IP/Western Blot R&D Proteome Profiler Human Phospho-Kinase Array Kit Contents: Each Human Phospho-Kinase Antibody Array Kit contains most of the necessary components for a ready-to-run assay without the need for specialized equipment.* • 8 (4 of membrane A and 4 of membrane B)-Single Use Nitrocellulose Membranes spotted in duplicate with capture and control antibodies • 8-Well Multi-dish • Lysis Buffer 6 • Array Buffer 1 (for blocking and sample incubation) • Array Buffer 2/3 (for dilution of the detection antibodies) • Wash Buffer Concentrate, 25X (for washing the arrays after lysate and detection antibody incubations) • Anti-Phospho-Kinase Detection Antibody Cocktail A • Anti-Phospho-Kinase Detection Antibody Cocktail B • Streptavidin-HRP • Transparency Overlay Template • Detailed product insert with protocol