SelectScience
Join Free
Accelerating Science
NewsLife Sciences

R&D Systems Presents New Tools for Profiling the Phosphorylation of Multiple Signaling Molecules

Bio-Techne
27 Mar 2011
bridget bridget
Laboratory Director

Product news

Proteome Profiler 96™ Arrays allow researchers to measure the relative phosphorylation of up to 16 kinases in the single well of a 96 well plate. Because standard chemiluminescence cameras are used for data collection, many laboratories bypass the need for expensive, specialized equipment.

Stop by our booth (#467) for promotions and to find out more about Proteome Profiler 96, including our newest array designed to simultaneously measure the phosphorylation of Akt, ERK1/ERK2, GSK-3ß, JNK, p38-alpha, p70 S6 Kinase, and Src.

Proteome Profiler 96 Antibody Arrays are 96-well microplate-based assays designed to detect multiple proteins in the same sample. Microplate wells are spotted with up to 16 different capture antibodies allowing highly efficient analysis of relative protein expression levels. These kits use a smaller sample size than our membrane-based Proteome Profiler Antibody Arrays. Proteome Profiler 96 Antibody Arrays are specially designed to ensure antibody compatibility, high specificity, and high sensitivity. Each kit contains a detection antibody and all required buffers necessary to run the assay.

Related products

Request Quote for All Products

Proteome Profiler Antibody Arrays

Bio-Techne

R&D Proteome Profiler Antibody Arrays R&D Proteome Profiler Arrays allow for the measurement of multiple proteins in a single sample. These arrays require no specialized equipment, and eliminate the need for multiple immunoprecipitation/Western blot experiments. R&D Array kits contain buffers, detection antibodies, and membranes spotted in duplicate with capture antibodies carefully selected for their specificity. R&D Arrays include:R&D Systems Human Phospho-RTK Antibody Array (Catalog # ARY001)Detect the relative site-specific phosphorylation levels of 42 different receptor tyrosine kinases(RTKs). R&D Systems Human Phospho-MAPK Antibody Array (Catalog # ARY002) Detect the relative site-specific phosphorylation levels of 19 different intracellular serine/threonine kinases. R&D Systems Human Phospho-Kinase Antibody Array (Catalog # ARY003) Detect the relative phosphorylation levels of 46 intracellular serine/threonine/tyrosine kinases. R&D Systems Human Phospho-Immunoreceptor Antibody Array (Catalog # ARY004) Detect the relative phosphorylation levels of 59 ITAM/ITIM-associated immunoreceptors. R&D Systems Human Cytokine Antibody Array, Panel A (Catalog # ARY005) Detect the relative levels of 36 cytokines, chemokines, and acute phase proteins simultaneously. R&D Systems Mouse Cytokine Antibody Array, Panel A (Catalog # ARY006) Detect the relative levels of 40 cytokines, chemokines, and more simultaneously. R&D Systems Human Angiogenesis Antibody Array (Catalog # ARY007) Detect the relative levels of 55 angiogenesis-related proteins simultaneously. R&D Systems Rat Cytokine Antibody Array (Catalog # ARY008) Detect the relative levels of 29 cytokines, chemokines and more simultaneously. R&D Systems Human Apoptosis Antibody Array (Catalog # ARY009) Detect the relative levels of 35 apoptosis-related proteins simultaneously. R&D Systems Human Pluripotent Stem Cell Antibody Array (Catalog # ARY010) Detect the relative levels of 15 pluripotent stem cell markers simultaneously.

(1)

Links

Bio-TechneCompany website

Tags

AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Cell Adhesion AssaysCell adhesion assays are used to quantitate attachment and analyze the molecular mechanisms for extracellular matrix adhesion, cell migration and sensitivity to inhibitors. Find the best cell adhesion assay equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ADME-ToxicologyADME-toxicology (ADME-Tox) studies are used in pharmacology and pharmacokinetics to assess the activity/toxicity of drugs <i>in vivo</i> or <i>in vitro</i>. Find bioassays for absorption, distribution, metabolism, and excretion of drug molecules including cytotoxicity, transporter/permeability, metabolism and activity assays as well as hepatocytes and cell lines for ADME. Find the best ADME-toxicology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Assay AssemblyAssay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

Related articles

R&D Systems Presents New Tools for Profiling the Phosphorylation of Multiple Signaling Molecules