NewsLife Sciences
Quick & Efficient Native ChIP Protocol from Chromatrap
7 Apr 2016Alex Waite
Editorial Assistant
Industry news
Chromatrap, a division of Porvair Sciences, announces a new patented protocol that is able to simply, quickly and efficiently enrich transcription factors from Native Chromatin.
Native ChIP is the technique of choice for the study of histone modifiers and certain abundant transcription factors that are likely to be bound to DNA.
Traditionally Native ChIP assays have involved five key time-consuming steps:
- Preparation of chromatin to be analysed from cells
- Immunoprecipitation of chromatin using a high quality ChIP-validated antibody specific to the target protein
- isolation of the precipitated chromatin fragments
- DNA recovery from the precipitated product
- DNA analysis.
The Chromatrap® N-ChIP kit provides a new quicker, easier and more efficient way of performing N-ChIP assays (Patent No. GB2482209). It uses discs of an inert, porous, polymer to which Protein A or Protein G has been covalently attached to maximize the capture efficiency of the target chromatin/antibody complex.
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